Abstract | AIMS: MAIN METHODS: Adult male Sprague-Dawley rats were divided in standard diet (SD) or HFD, with sedentary, VPA and ET animals in both diet regimens. Plasma glucose and insulin concentrations were analyzed; plasma insulin sensitivity index (ISI) was calculated. Hepatic insulin, pro-inflammatory and glucocorticoid signaling regulators/mediators were evaluated by Western blot or reverse transcriptase-PCR. KEY FINDINGS: ET improved ISI in both diet regimens. HFD-feeding increased interleukin-1β and induced a similar pattern on interleukin-6 and TGF-β, which were globally reduced by physical exercise. ET decreased HFD leukemia inhibitory factor level, SD+VPA animals presenting higher values than HFD+VPA animals. HFD increased the ratio of IRS-1(Ser307)/total IRS-1, which was completely mitigated by physical exercise. Physical exercise reduced total ERK and JNK (total and activated) expression in HFD. In SD vs. HFD, VPA presented higher activated JNK and ET presented higher total JNK. Generally, in HFD, the ratio (activated/total) of AKT, and each separately, decreased with exercise and also for activated AKT in SD. Overall, in both diets, exercise reduced 11β-hydroxysteroid dehydrogenase type 1. ET increased glucocorticoid receptor and reduced PTP1B in HFD. SIGNIFICANCE: Physical exercise mitigates the expression of pro-inflammatory mediators and positively modulates insulin and glucocorticoid signaling in NASH.
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Authors | E Passos, C D Pereira, I O Gonçalves, S Rocha-Rodrigues, N Silva, J T Guimarães, D Neves, A Ascensão, J Magalhães, M J Martins |
Journal | Life sciences
(Life Sci)
Vol. 123
Pg. 51-60
(Feb 15 2015)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 25534442
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Inc. All rights reserved. |
Chemical References |
- DNA Primers
- Glucocorticoids
- Inflammation Mediators
- Insulin
- Transforming Growth Factor beta
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Topics |
- Animals
- Blotting, Western
- DNA Primers
(genetics)
- Diet, High-Fat
(adverse effects)
- Glucocorticoids
(metabolism)
- Inflammation Mediators
(metabolism)
- Insulin
(metabolism)
- Insulin Resistance
(physiology)
- Male
- Motor Activity
(physiology)
- Non-alcoholic Fatty Liver Disease
(complications, etiology, physiopathology)
- Physical Conditioning, Animal
(physiology)
- Physical Endurance
(physiology)
- Rats
- Rats, Sprague-Dawley
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction
(physiology)
- Statistics, Nonparametric
- Transforming Growth Factor beta
(metabolism)
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