β-D-Glucan nanoparticle pre-treatment induce resistance against Pythium aphanidermatum infection in turmeric.

Abstract

In vitro experiments were carried out to test the efficacy of GNP (β-D-glucan nanoparticle prepared from mycelium of Pythium aphanidermatum) against rhizome rot disease of turmeric (Curcuma longa L.) caused by P. aphanidermatum. GNP (0.1%, w/v) was applied to rhizome prior to inoculation with P. aphanidermatum (0 h, 24 h). Cell death, activities of defense enzymes such as peroxidase, polyphenol oxidase, protease inhibitor and β-1,3 glucanase were monitored. Prior application of GNP (24 h) to turmeric rhizome effectively controls P. aphanidermatum infection. The increase in defense enzyme activities occurred more rapidly and was enhanced in P. aphanidermatum infected rhizomes that were pre-treated with GNP. Pre-treatment also induced new isoforms of defense enzymes. Increased activities of defense enzymes suggest that they play a key role in restricting the development of disease symptoms in the rhizomes as evidenced by a reduction in cell death. The results demonstrated that GNP can be used as a potential agent for control of rhizome rot disease.

• Authors: Sathiyanarayanan Anusuya, Muthukrishnan Sathiyabama
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 74 Pg. 278-82 (Mar 2015) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 25524742 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• beta-Glucans
• Catechol Oxidase
• Peroxidase

Topics

• Catechol Oxidase (metabolism)
• Cell Death (drug effects)
• Curcuma (drug effects, metabolism, microbiology)
• Enzyme Activation (drug effects)
• Mycelium (chemistry)
• Nanoparticles (chemistry)
• Peroxidase (metabolism)
• Plant Diseases (microbiology)
• Pythiosis (drug therapy, microbiology)
• Pythium (chemistry)
• beta-Glucans (administration & dosage, chemistry)