High plasma pancreastatinlike immunoreactivity in a patient with malignant insulinoma.

Abstract

High levels of pancreastatinlike immunoreactivity were detected in the plasma (2.9 pmol/ml, greater than 200-fold the normal level), pancreas (2.9 nmol/g wet wt, greater than 450-fold the normal level), and liver (1.6 nmol/g wet wt) of a patient with pancreatic insulinoma with metastasis to the liver by a sensitive and specific radioimmunoassay for human pancreastatin. Antiserum was produced against the C-terminal fragment of human pancreastatin-(24-52), which was synthesized according to the sequence of human chromogranin A corresponding to that of pancreastatin. With the antiserum, intense immunocytochemical staining was detected in the tumors. Sephadex G-50 gel filtration showed that the tumors and plasma contained two molecular forms of pancreastatinlike immunoreactivity--a molecular form coeluted with synthetic human pancreastatin-52 and a larger molecular form (Mr approximately 12,000-15,000). The smaller form eluted in the same position as synthetic human pancreastatin-52 on reverse-phase high-performance liquid chromatography.

• Authors: K Tateishi, A Funakoshi, A Jimi, S Funakoshi, H Tamamura, H Yajima, Y Matsuoka
• Journal: Gastroenterology (Gastroenterology) Vol. 97 Issue 5 Pg. 1313-8 (Nov 1989) ISSN: 0016-5085 [Print] United States
• PMID: 2551767 (Publication Type: Case Reports, Journal Article)

Chemical References

• Chromogranin A
• Pancreatic Hormones
• pancreastatin

Topics

• Adenoma, Islet Cell (analysis)
• Chromatography, High Pressure Liquid
• Chromogranin A
• Female
• Humans
• Insulinoma (analysis, secondary)
• Liver (analysis)
• Liver Neoplasms (analysis, secondary)
• Middle Aged
• Pancreas (analysis)
• Pancreatic Hormones
• Pancreatic Neoplasms (analysis)
• Radioimmunoassay (methods)