Abstract | AIM: The aim of this study was to evaluate the osteoprotective effect of aqueous Rhizoma Dioscoreae extract (RDE) on the alveolar bone of rats with ovariectomy-induced bone loss. METHODS: Female Wistar rats were subjected to either ovariectomy or a sham operation ( SHAM). The ovariectomized (OVX) rats were treated with vehicle (OVX) or RDE by oral gavage or with 17β-estradiol (E2) subcutaneously. After treatments, the bone mineral density (BMD), the three-dimensional bone architecture of the alveolar bone and the plasma biomarkers of bone turnover were analyzed to assess bone metabolism, and the histomorphometry of the alveolar bone was observed. Microarrays were used to evaluate gene expression profiles in alveolar bone from RDE-treated and OVX rats. The differential expression of genes was further analyzed using Ingenuity Pathway Analysis (IPA). The key findings were verified using real-time quantitative RT-PCR (qRT-PCR). RESULTS: Our results showed that RDE inhibited alveolar bone loss in OVX rats. Compared to the OVX rats, the RDE-treated rats showed upregulated expression levels of 207 genes and downregulated expression levels of 176 genes in the alveolar bone. The IPA showed that several genes had the potential to code for proteins that were involved in the Wnt/β- catenin signaling pathway (Wnt7a, Fzd2, Tcf3, Spp1, Frzb, Sfrp2 and Sfrp4) and the p38 MAPK signaling pathway (Il1rn and Mapk14). CONCLUSION: These experiments revealed that RDE could inhibit ovariectomy-induced alveolar bone loss in rats. The mechanism of this anti-osteopenic effect in alveolar bone may be involved in the reduced abnormal bone remodeling, which is associated with the modulation of the Wnt/β- catenin and the p38 MAPK signaling pathways via gene regulation.
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Authors | Zhiguo Zhang, Lihua Xiang, Dong Bai, Wenlai Wang, Yan Li, Jinghua Pan, Hong Liu, Shaojun Wang, Gary Guishan Xiao, Dahong Ju |
Journal | Nutrients
(Nutrients)
Vol. 6
Issue 12
Pg. 5853-70
(Dec 12 2014)
ISSN: 2072-6643 [Electronic] Switzerland |
PMID | 25514564
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Collagen Type I
- Ctnnb1 protein, rat
- Peptide Fragments
- Plant Extracts
- Procollagen
- Wnt Proteins
- beta Catenin
- procollagen Type I N-terminal peptide
- Estradiol
- p38 Mitogen-Activated Protein Kinases
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Topics |
- Alveolar Bone Loss
(drug therapy)
- Animals
- Biomarkers
(blood)
- Bone Density
(drug effects)
- Bone Diseases, Metabolic
(drug therapy, etiology)
- Bone Remodeling
(drug effects)
- Collagen Type I
(blood)
- Dioscorea
(chemistry)
- Down-Regulation
- Estradiol
(blood, pharmacology)
- Female
- MAP Kinase Signaling System
- Ovariectomy
- Peptide Fragments
(blood)
- Plant Extracts
(pharmacology)
- Procollagen
(blood)
- Rats
- Rats, Wistar
- Rhizome
(chemistry)
- Signal Transduction
- Up-Regulation
- Wnt Proteins
(genetics, metabolism)
- Wnt Signaling Pathway
- beta Catenin
(genetics, metabolism)
- p38 Mitogen-Activated Protein Kinases
(genetics, metabolism)
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