Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: MATERIALS AND METHODS:
Ethyl acetate extract (SLE), methanolic extract (SLM) and aqueous extract (SLW) were evaluated in vitro on XO inhibitory activity and in vivo in an experimental model with oxonate-induced hyperuricemia in mice which was used to evaluate anti-hyperuricemic activity and liver xanthine oxidase (XOD) inhibition. Anti-inflammatory activity was also investigated on MSU crystal-induced paw edema model. RESULTS: Sparattosperma leucanthum crude extracts showed expressive results on urate-lowering activity in blood. SLW at the dose of 125 mg/kg has proved to be active in reducing hyperuricemia and was capable to inhibit the hepatic xanthine oxidase enzyme (XOD). SLM showed anti-hyperuricemic activity on all doses tested; however, this extract showed activity on the XOD only at the dose of 500 mg/kg. SLE, at the three evaluated doses, has proved to be active in reducing hyperuricemia in vivo and was able to inhibit XO activity in vitro at the concentration of 100 µg/mL. This extract was also able to inhibit XOD activity in vivo at the doses of 250 mg/kg and 500 mg/kg. SLE (125 and 250 mg/kg) and SLW (500 mg/kg) showed significant anti-inflammatory activity on monosodium urate crystal-induced paw edema model. CONCLUSIONS: The ethyl acetate, methanolic and aqueous extracts of Sparattosperma leucanthum showed significant results on evaluated models and therefore may be important agents for the treatment of gouty arthritis and hyperuricemia.
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Authors | Rita de Cássia Lemos Lima, Fernanda Cristina Ferrari, Maíra Ribeiro de Souza, Bruna Maria de Sá Pereira, Carmen Aparecida de Paula, Dênia Antunes Saúde-Guimarães |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 161
Pg. 194-9
(Feb 23 2015)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 25500302
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Gout Suppressants
- Plant Extracts
- Uric Acid
- Oxonic Acid
- Xanthine Oxidase
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Topics |
- Animals
- Anti-Inflammatory Agents
(pharmacology, therapeutic use)
- Arthritis, Gouty
(blood, drug therapy)
- Bignoniaceae
- Gout Suppressants
(pharmacology, therapeutic use)
- Hyperuricemia
(blood, chemically induced, drug therapy)
- Liver
(drug effects, metabolism)
- Male
- Mice
- Oxonic Acid
- Phytotherapy
- Plant Extracts
(pharmacology, therapeutic use)
- Plant Leaves
- Uric Acid
(blood)
- Xanthine Oxidase
(antagonists & inhibitors, metabolism)
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