Dopamine treatment of brain-dead Fisher rats improves renal histology but not early renal function in Lewis recipients after prolonged static cold storage.

Abstract

BACKGROUND:

Brain death (BD) and cold preservation are major risk factors for an unfavorable transplantation outcome. Although donor dopamine treatment in brain-dead rats improves renal function and histology in allogeneic recipients, it remains to be assessed if this also holds true for the combinations of BD and prolonged static cold preservation.

METHODS:

BD was induced in F344 donor rats, which were subsequently treated with NaCl 1 mL/h (BD, n = 11), NaCl/hydroxy ethyl starch (BD-norm, n = 10), or 10 μg/min/kg dopamine (BD-dopa, n = 10). Renal grafts were harvested 4 h after BD and transplanted into bilateral nephrectomized Lewis recipients 6 h after cold preservation in University of Wisconsin solution. Renal function was evaluated by use of serum creatinine and urea concentrations at days 0, 1, 3, 5, and 10. Ten days after transplantation, recipients were killed and the renal allografts were processed for light microscopy and immune histology.

RESULTS:

Serum urea concentrations at days 5 and 10 were significantly lower in recipients that received a renal graft from dopamine-treated rats; for serum creatinine, only a trend was observed at day 10. Immune histology revealed a lower degree of ED1-positive cells in the donor dopamine-treated group. Under light microscopy, Banff classification revealed significantly less intimal arteritis in these grafts (P < .05).

CONCLUSIONS:

Although donor dopamine treatment clearly improves renal histology in this model, the beneficial effect on early renal function was marginal. It remains to be assessed if donor dopamine treatment has a beneficial effect on renal function in long-term follow-up.

• Authors: J Fontana, B Yard, E Stamellou, H Wenz, U Benck, P Schnuelle, S Hoeger
• Journal: Transplantation proceedings (Transplant Proc) Vol. 46 Issue 10 Pg. 3319-25 (Dec 2014) ISSN: 1873-2623 [Electronic] United States
• PMID: 25498044 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Cardiotonic Agents
• Insulin
• Organ Preservation Solutions
• University of Wisconsin-lactobionate solution
• Allopurinol
• Glutathione
• Adenosine
• Raffinose
• Dopamine

Topics

• Adenosine (pharmacology)
• Allopurinol (pharmacology)
• Animals
• Brain Death
• Cardiotonic Agents (pharmacology)
• Disease Models, Animal
• Dopamine (pharmacology)
• Follow-Up Studies
• Glomerular Filtration Rate (drug effects, physiology)
• Glutathione (pharmacology)
• Insulin (pharmacology)
• Kidney (drug effects, pathology, physiopathology)
• Kidney Transplantation (methods)
• Male
• Organ Preservation Solutions (pharmacology)
• Raffinose (pharmacology)
• Rats
• Rats, Inbred F344
• Rats, Inbred Lew
• Tissue Donors
• Transplantation, Homologous