Abstract | INTRODUCTION: METHODS: In this study, taking advantage of the transparency of larval zebrafish, we developed a zebrafish hyperlipidemia model for drug screening and efficacy assessment. Zebrafish at 5 d.p.f (days post fertilization) were fed with 0.1% egg yolk for 48 h (hours), followed by drug treatment for 24h or 48 h. Tested drugs were administered into the zebrafish by direct soaking. Drug effect was evaluated based on quantitative analysis of Oil Red O (ORO) in zebrafish vena caudalis. RESULTS: DISCUSSION:
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Authors | Juan Zhou, Yi-Qiao Xu, Sheng-Ya Guo, Chun-Qi Li |
Journal | Journal of pharmacological and toxicological methods
(J Pharmacol Toxicol Methods)
2015 Mar-Apr
Vol. 72
Pg. 47-52
ISSN: 1873-488X [Electronic] United States |
PMID | 25497901
(Publication Type: Journal Article)
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Copyright | Copyright © 2014. Published by Elsevier Inc. |
Chemical References |
- Azo Compounds
- Coloring Agents
- Hypolipidemic Agents
- oil red O
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Topics |
- Animals
- Azo Compounds
- Beverages
- Biological Assay
(methods)
- Coloring Agents
- Dose-Response Relationship, Drug
- Hypolipidemic Agents
(pharmacology)
- Larva
(drug effects)
- Medicine, Chinese Traditional
- Staining and Labeling
- Time
- Zebrafish
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