We investigated whether attenuating
dipeptidyl peptidase-IV (DPP4)
enzyme activity protected rat heart from
ischemia-reperfusion (IR) injury (40-min left anterior descending coronary artery
ligation followed by 72 h reperfusion).
METHODS AND RESULTS: Adult male Fischer 344 rats (n = 24) were equally divided into
sham-control (WT-SC), WT-IR, and WT-IR-Sita (oral
sitagliptin 400 mg/kg/day for 3 days) groups, whereas adult male DPP4-deficiency (DPP4(D)) rats (n = 16) were equally divided into DPP4(D)-SC and DPP4(D)-IR groups. Animals were sacrificed at 72 h after reperfusion with collection of heart specimens.
Infarct area (H&E),
collagen deposition (Sirius-red
stain), fibrotic area (Masson's trichrome), and fluorescent-ROS intensity (H2DCFDA-labeling myocardium) of left ventricle were significantly higher in WT-IR than those in other groups, significantly higher in WT-IR-Sita and DPP4(D)-IR groups than in WT-SC and DPP4(D)-SC groups (all p < 0.001), but there was no difference between the latter two groups.
Protein expressions of oxidative stress (oxidized
protein),
reactive oxygen species (NOX-1, NOX-2),
inflammation (TNF-α, NF-κB, MMP-9, VCAM-1), apoptosis (mitochondrial Bax, cleaved
caspase-3 and PARP), myocardial damage markers (cytosolic cytochrome-C, γ-H2AX), and number of inflammatory cells (CD14+, CD68+, CD40+ cells) showed a pattern identical to that of histological changes among all groups (all p < 0.005), whereas markers of anti-apoptosis (Bcl-2) and mitochondrial integrity (mitochondrial
cytochrome-C) as well as left ventricular ejection fraction showed an opposite pattern (all p < 0.001).
Protein expressions of
anti-oxidants (HO-1, NQO-1), angiogenesis factors (SDF-1α, CXCR4), and glycogen-like-peptide-1-receptor were significantly higher inWT-IR-Sita and DPP4(D)-IR than those in other groups (all p <0.001).
CONCLUSION: Abrogation of DPP4 activity protects against myocardial IR injury and preserved heart function.