Abstract | INTRODUCTION: METHODS: Subjects with deep carious lesions with or without periapical lesions (≥3 mm) were recruited at the University of Pittsburgh, Pittsburgh, PA, and the University of Texas at Houston, Houston, TX. Genomic DNA samples of 316 patients were sorted into 2 groups: 136 cases with deep carious lesions and periapical lesions (cases) and 180 cases with deep carious lesions but no periapical lesions (controls). Nine single-nucleotide polymorphisms in IL1B, IL6, TNF, RANK, RANKL, and OPG genes were selected for genotyping. Genotypes were generated by end point analysis using TaqMan chemistry (Invitrogen, Carlsbad, CA) in a real-time polymerase chain reaction instrument. Allele and genotype frequencies were compared among cases and controls using the PLINK program (http://pngu.mgh.harvard.edu/purcell/plink/). Ninety-three human periapical granulomas and 24 healthy periodontal ligament tissues collected postoperatively were used for messenger RNA expression analyses of IL1B. RESULTS: A single-nucleotide polymorphism in IL1B (rs1143643) showed allelic (P = .02) and genotypic (P = .004) association with cases of deep caries and periapical lesions. We also observed altered transmission of IL1B marker haplotypes (P = .02) in these individuals. IL1B was highly expressed in granulomas (P < .001). CONCLUSIONS: Variations in IL1B may be associated with periapical lesion formation in individuals with untreated deep carious lesions. Future studies could help predict host susceptibility to developing periapical lesions.
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Authors | Alisa Dill, Ariadne Letra, Letícia Chaves de Souza, Mamatha Yadlapati, Claudia Cristina Biguetti, Gustavo Pompermaier Garlet, Alexandre R Vieira, Renato Menezes Silva |
Journal | Journal of endodontics
(J Endod)
Vol. 41
Issue 2
Pg. 197-200
(Feb 2015)
ISSN: 1878-3554 [Electronic] United States |
PMID | 25476976
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- IL1B protein, human
- Interleukin-1beta
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Topics |
- Adult
- Aged
- Alleles
- Dental Caries
(genetics, physiopathology)
- Female
- Genetic Association Studies
- Genetic Predisposition to Disease
- Genotype
- Humans
- Interleukin-1beta
(genetics)
- Male
- Middle Aged
- Periapical Abscess
(genetics, physiopathology)
- Periapical Periodontitis
(genetics, physiopathology)
- Polymorphism, Single Nucleotide
- Tooth Apex
(physiopathology)
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