Abstract | AIM: METHODS: ICR mice were randomly divided into six groups (Group 1: normal; Group 2: CCl4-treated group; Group 3: CCl4 plus ursolic acid 25mg/kg group; Group 4: CCl4 plus ursolic acid 50mg/kg group; Group 5: CCl4 plus colchicine 1mg/kg group; Group 6: ursolic acid 50mg/kg group). Mice were administered with CCl4 (2 mL of CCl4 in olive oil (1:1, v/v) per kg body weight twice weekly) by intraperitoneal injection and oral injection of colchicine (1mg/kg) or ursolic acid (25, 50mg/kg) daily. After six weeks, serum aminotransferase activity, hepatic reactive oxygen species (ROS) production, thiobarbituric acid reactive substances ( TBARS), antioxidase (SOD, CAT, GPx) activity and histopathological analysis were performed. The levels of nuclear factor E2-related factor 2 (Nrf2), NAD(P)H: quinone oxidoreductase-1 (NQO1), glutathione S-transferase (GST) and heme oxygenase-1 (HO-1), tumor necrosis factor-alpha (TNF-α), prostaglandin E2 ( PGE2) and inducible nitric oxide synthase (iNOS), Bcl-2 and caspase-3 were measured. RESULTS:
Ursolic acid significantly prevented CCl4-induced hepatotoxicity and fibrosis, indicated by both diagnostic indicators and histopathological analysis. CCl4-induced profound elevations of oxidative stress, inflammation and apoptosis in liver were suppressed by ursolic acid. CONCLUSIONS:
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Authors | Jie-Qiong Ma, Jie Ding, Li Zhang, Chan-Min Liu |
Journal | Clinics and research in hepatology and gastroenterology
(Clin Res Hepatol Gastroenterol)
Vol. 39
Issue 2
Pg. 188-97
(Apr 2015)
ISSN: 2210-741X [Electronic] France |
PMID | 25459994
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Masson SAS. All rights reserved. |
Chemical References |
- NF-E2-Related Factor 2
- Nfe2l2 protein, mouse
- Triterpenes
- ursolic acid
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Topics |
- Animals
- Antioxidant Response Elements
(physiology)
- Disease Models, Animal
- Liver Cirrhosis
(prevention & control)
- Male
- Mice
- NF-E2-Related Factor 2
(physiology)
- Random Allocation
- Signal Transduction
- Triterpenes
(therapeutic use)
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