Abstract |
Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or inactivated whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 lysate vaccine adjuvanted with Iscom Matrix to generate a longer lasting specific antibody response in blood and milk, with improved opsonic capacity, compared with a S. aureus CP5 whole-cell formulation. The aim of the present study was to obtain an experimental immunogen composed of lysed cells of a CP5 S. aureus strain supplemented with recombinant clumping factor A, fibronectin binding protein A and β-toxin formulated with Iscom Matrix, characterize the immune response generated when immunizing pregnant heifers and assess the functional role of antibodies raised against this immunogen in experimental models. Both a lysate vaccine and a lysate+recombinant antigens vaccine elicited antibodies that promoted neutrophil phagocytosis and inhibited internalization into mammary epithelial cells, in vitro. Incorporation of defined antigenic molecules to the lysate formulation elicited a strong specific humoral immune response against both lysate and recombinant antigens and was associated with higher expression of regulatory and pro-inflammatory cytokines. In addition, antibodies were efficient for blocking S. aureus binding to bovine fibrinogen and fibronectin, and neutralizing β-toxin effect in vitro, placing these antigens as candidates to be included in a formulation directed to prevent staphylococcal bovine mastitis.
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Authors | C M Camussone, N Pujato, M S Renna, C M Veaute, B Morein, I S Marcipar, L F Calvinho |
Journal | Veterinary immunology and immunopathology
(Vet Immunol Immunopathol)
Vol. 162
Issue 3-4
Pg. 96-107
(Dec 15 2014)
ISSN: 1873-2534 [Electronic] Netherlands |
PMID | 25454469
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier B.V. All rights reserved. |
Chemical References |
- Adhesins, Bacterial
- Antibodies, Bacterial
- Bacterial Toxins
- ClfA protein, Staphylococcus aureus
- Coagulase
- Cytokines
- Hemolysin Proteins
- ISCOMs
- Staphylococcal Vaccines
- Vaccines, Synthetic
- fibronectin-binding proteins, bacterial
- Sphingomyelin Phosphodiesterase
- hlb protein, Staphylococcus aureus
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Topics |
- Adhesins, Bacterial
(genetics, immunology)
- Animals
- Antibodies, Bacterial
(blood)
- Bacterial Toxins
(genetics, immunology)
- Cattle
- Cell Line
- Coagulase
(genetics, immunology)
- Cytokines
(blood)
- Female
- Hemolysin Proteins
(genetics, immunology)
- ISCOMs
(pharmacology)
- Immunization
(methods, veterinary)
- Mastitis, Bovine
(immunology, microbiology, prevention & control)
- Milk
(microbiology)
- Pregnancy
- Random Allocation
- Sphingomyelin Phosphodiesterase
(genetics, immunology)
- Staphylococcal Infections
(immunology, microbiology, prevention & control, veterinary)
- Staphylococcal Vaccines
(administration & dosage, immunology)
- Staphylococcus aureus
(immunology)
- Statistics, Nonparametric
- Vaccines, Synthetic
(administration & dosage, immunology, standards)
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