Peptide imprinted receptors for the determination of the small cell lung cancer associated biomarker progastrin releasing peptide.

Abstract

Peptide imprinted polymers were developed for detection of progastrin releasing peptide (ProGRP); a low abundant blood based biomarker for small cell lung cancer. The polymers targeted the proteotypic nona-peptide sequence NLLGLIEAK and were used for selective enrichment of the proteotypic peptide prior to LCMS based quantification. Peptide imprinted polymers with the best affinity characteristics were first identified from a 96-polymer combinatorial library. The effects of functional monomers, crosslinker, porogen, and template on adsorption capacity and selectivity for NLLGLIEAK were investigated and optimized. Ultimately, a solid phase extraction method was developed for highly selective enrichment of the target peptide from tryptic digests.

• Authors: Abed Abdel Qader, Javier Urraca, Silje Bøen Torsetnes, Finn Tønnesen, Léon Reubsaet, Börje Sellergren
• Journal: Journal of chromatography. A (J Chromatogr A) Vol. 1370 Pg. 56-62 (Nov 28 2014) ISSN: 1873-3778 [Electronic] Netherlands
• PMID: 25454129 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Biomarkers, Tumor
• Gastrins
• Peptides
• Protein Precursors
• big gastrin

Topics

• Adsorption
• Amino Acid Sequence
• Biomarkers, Tumor (analysis)
• Gastrins (metabolism)
• Humans
• Lung Neoplasms (chemistry)
• Molecular Imprinting (methods)
• Peptides (chemistry)
• Protein Precursors (metabolism)
• Small Cell Lung Carcinoma (chemistry)
• Solid Phase Extraction (methods)