Synthesis and characterization of pectin derivative with antitumor property against Caco-2 colon cancer cells.

Abstract

New pectin derivative (Pec-MA) was obtained in specific reaction conditions. The presence of maleoyl groups in Pec-MA structure was confirmed by (1)H NMR and FTIR spectroscopy. The substitution degree of Pec-MA (DS=24%) was determined by (1)H NMR. The properties of Pec-MA were investigated through WAXS, TGA/DTG, SEM and zeta potential techniques. The Pec-MA presented amorphous characteristics and higher-thermal stability compared to raw pectin (Pec). In addition, considerable morphological differences between Pec-MA and Pec were observed by SEM. The cytotoxic effect on the Caco-2 cells showed that the Pec-MA significantly inhibited the growth of colon cancer cells whereas the Pec-MA does not show any cytotoxic effect on the VERO healthy cells. This result opens new perspectives for the manufacture of biomaterials based on Pec with anti-tumor properties.

• Authors: Elizângela A M S Almeida, Suelen P Facchi, Alessandro F Martins, Samara Nocchi, Ivânia T A Schuquel, Celso V Nakamura, Adley F Rubira, Edvani C Muniz
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 115 Pg. 139-45 (Jan 22 2015) ISSN: 1879-1344 [Electronic] England
• PMID: 25439878 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Antineoplastic Agents
• Pectins

Topics

• Animals
• Antineoplastic Agents (chemical synthesis, chemistry, pharmacology)
• Caco-2 Cells
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Chlorocebus aethiops
• Colonic Neoplasms (drug therapy, pathology)
• Dose-Response Relationship, Drug
• Drug Screening Assays, Antitumor
• Humans
• Molecular Structure
• Pectins (chemical synthesis, chemistry, pharmacology)
• Structure-Activity Relationship
• Vero Cells