Increased phosphoinositide breakdown by phospholipase C in erythrocyte membranes from patients with cystic fibrosis.

Abstract

Phosphoinositide phospholipase C activity has been measured in erythrocyte membranes from age-matched control and CF subjects. Inositol phospholipids were labelled with [3H]myo-inositol and control experiments demonstrated that the [3H]-labelled products released by incubation of membranes with Ca2+ were derived specifically from erythrocytes (a) by purification of erythrocytes on cellulose columns, (b) by demonstration that the phospholipase C activity was inhibited by 10 mmol/l neomycin but not by 1 mmol/l p-methylsulphonylfluoride. The [3H]-labelled products were shown to be inositol phosphates by their elution from anion-exchange columns. Membranes from CF patients showed increased phospholipase C activity compared to controls which did not correlate with the degree of [3H]inositol labelling of the membranes, with pancreatic function as assessed by serum immunoreactive trypsin or with medications taken by the patients.

• Authors: C Doughney, M A McPherson, M C Goodchild, R L Dormer
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 181 Issue 1 Pg. 55-63 (Apr 28 1989) ISSN: 0009-8981 [Print] Netherlands
• PMID: 2541951 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Phosphatidylinositols
• Sulfones
• methanesulfonyl fluoride
• Type C Phospholipases
• Neomycin
• Calcium

Topics

• Adolescent
• Adult
• Calcium (metabolism)
• Cystic Fibrosis (blood, metabolism)
• Erythrocyte Membrane (drug effects, enzymology)
• Female
• Humans
• Male
• Neomycin (pharmacology)
• Phosphatidylinositols (blood, metabolism)
• Sulfones (pharmacology)
• Type C Phospholipases (blood, metabolism)