Biofortified orange maize is as efficacious as a vitamin A supplement in Zambian children even in the presence of high liver reserves of vitamin A: a community-based, randomized placebo-controlled trial.

Abstract

BACKGROUND:

Biofortification is a strategy to relieve vitamin A (VA) deficiency. Biofortified maize contains enhanced provitamin A concentrations and has been bioefficacious in animal and small human studies.

OBJECTIVE:

The study sought to determine changes in total body reserves (TBRs) of vitamin A with consumption of biofortified maize.

DESIGN:

A randomized, placebo-controlled biofortified maize efficacy trial was conducted in 140 rural Zambian children. The paired (13)C-retinol isotope dilution test, a sensitive biomarker for VA status, was used to measure TBRs before and after a 90-d intervention. Treatments were white maize with placebo oil (VA-), orange maize with placebo (orange), and white maize with VA in oil [400 μg retinol activity equivalents (RAEs) in 214 μL daily] (VA+).

RESULTS:

In total, 133 children completed the trial and were analyzed for TBRs (n = 44 or 45/group). Change in TBR residuals were not normally distributed (P < 0.0001); median changes (95% CI) were as follows: VA-, 13 (-19, 44) μmol; orange, 84 (21, 146) μmol; and VA+, 98 (24, 171) μmol. Nonparametric analysis showed no statistical difference between VA+ and orange (P = 0.34); both were higher than VA- (P = 0.0034). Median (95% CI) calculated liver reserves at baseline were 1.04 (0.97, 1.12) μmol/g liver, with 59% >1 μmol/g, the subtoxicity cutoff; none were <0.1 μmol/g, the deficiency cutoff. The calculated bioconversion factor was 10.4 μg β-carotene equivalents/1 μg retinol by using the middle 3 quintiles of change in TBRs from each group. Serum retinol did not change in response to intervention (P = 0.16) but was reduced with elevated C-reactive protein (P = 0.0029) and α-1-acid glycoprotein (P = 0.0023) at baseline.

CONCLUSIONS:

β-Carotene from maize was efficacious when consumed as a staple food in this population and could avoid the potential for hypervitaminosis A that was observed with the use of preformed VA from supplementation and fortification. Use of more sensitive methods other than serum retinol alone, such as isotope dilution, is required to accurately assess VA status, evaluate interventions, and investigate the interaction of VA status and infection. This trial was registered at clinicaltrials.gov as NCT01814891.

• Authors: Bryan Gannon, Chisela Kaliwile, Sara A Arscott, Samantha Schmaelzle, Justin Chileshe, Ngándwe Kalungwana, Mofu Mosonda, Kevin Pixley, Cassim Masi, Sherry A Tanumihardjo
• Journal: The American journal of clinical nutrition (Am J Clin Nutr) Vol. 100 Issue 6 Pg. 1541-50 (Dec 2014) ISSN: 1938-3207 [Electronic] United States
• PMID: 25411289 (Publication Type: Journal Article, Randomized Controlled Trial, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 American Society for Nutrition.

Chemical References

• beta Carotene
• Vitamin A
• C-Reactive Protein

Topics

• C-Reactive Protein (metabolism)
• Child
• Diet
• Female
• Food, Fortified (analysis)
• Humans
• Liver (drug effects, metabolism)
• Male
• Vitamin A (administration & dosage, blood)
• Vitamin A Deficiency (blood, diet therapy, epidemiology)
• Zambia (epidemiology)
• Zea mays (chemistry)
• beta Carotene (administration & dosage)