Abstract | BACKGROUND/AIMS: Reports have suggested that the traditional Chinese medicine Smilacis Glabrae Rhizoma attenuates hyperuricemia, but its mechanism is unclear. Our previous study demonstrated that uric acid could induce the generation of reactive oxygen species(ROS), which subsequently cause endothelial dysfunction. Therefore, we focused on the oxidative stress process. In this study, we would use LC-MS and bioinformatic analysis to investigate the underlying mechanism. METHODS: We utilized LC-MS to reveal the differential protein expression in the kidneys of rats in the hyperuricemia group and the Smilacis Glabrae Rhizoma treatment group and then subjected the differentially expressed proteins to bioinformatic analysis. We also determined the serum ROS level of the two groups. According the above results, we built our hypothesis and performed in vitro experiments to validate this hypothesis. RESULTS: CONCLUSION: We demonstrated that Smilacis Glabrae Rhizoma could alleviate the oxidative stress caused by hyperuricemia by upregulating catalase expression. This finding could represent a new application for Smilacis Glabrae Rhizoma in the treatment of hyperuricemia.
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Authors | Quan Hong, Shandong Yu, Yan Mei, Yang Lv, Dapeng Chen, Yuanda Wang, Wenjia Geng, Di Wu, Guangyan Cai, Xiangmei Chen |
Journal | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
(Cell Physiol Biochem)
Vol. 34
Issue 5
Pg. 1675-85
( 2014)
ISSN: 1421-9778 [Electronic] Germany |
PMID | 25401709
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 S. Karger AG, Basel. |
Chemical References |
- Drugs, Chinese Herbal
- Reactive Oxygen Species
- Uric Acid
- Catalase
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Topics |
- Animals
- Catalase
(metabolism)
- Computational Biology
(methods)
- Drugs, Chinese Herbal
(chemistry, pharmacology)
- Endothelial Cells
(drug effects, metabolism)
- Hyperuricemia
(complications, metabolism)
- Kidney
(drug effects, metabolism)
- Medicine, Chinese Traditional
(methods)
- Oxidative Stress
(drug effects)
- Rats
- Rats, Sprague-Dawley
- Reactive Oxygen Species
(metabolism)
- Rhizome
(chemistry)
- Up-Regulation
(drug effects)
- Uric Acid
(metabolism)
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