Polyethylene glycol-attached antioxidant enzymes decrease pulmonary oxygen toxicity in rats.

Abstract

When exposed continuously to hyperoxia (100% O2, 760 Torr barometric pressure), rats pretreated with polyethylene glycol (PEG)-attached superoxide dismutase and catalase (PEG-SOD + PEG-CAT) lived longer (79.1 + 7.6 h) than rats pretreated with saline (60.7 +/- 2.1 h) or PEG-inactivated-SOD + PEG-inactivated-CAT (62.3 +/- 1.6 h). Rats pretreated with PEG-SOD + PEG-CAT also had less hyperoxia-induced acute oxidative edematous lung injury, as assessed by increases in lung oxidized glutathione (GSSG) contents, pleural effusions, and lung lavage albumin concentrations than saline-pretreated rats. Rats pretreated with the long-lived conjugates PEG-inactivated-SOD + PEG-inactivated-CAT or PEG-albumin also had decreased acute oxidative edematous lung injury compared with rats pretreated with PEG, SOD + CAT + PEG, SOD + CAT, or saline. In vitro studies suggested that PEG itself may have contributed to protection by scavenging hydroxyl radical (.OH) but not superoxide (O2-.) or H2O2. Compared with more effective endogenous (via preexposure to hypoxia) or exogenous (via liposomes) means for increasing lung antioxidant enzymes, PEG enzymes are less protective against lung injury from continuous hyperoxia.

• Authors: C W White, J H Jackson, A Abuchowski, G M Kazo, R F Mimmack, E M Berger, B A Freeman, J M McCord, J E Repine
• Journal: Journal of applied physiology (Bethesda, Md. : 1985) (J Appl Physiol (1985)) Vol. 66 Issue 2 Pg. 584-90 (Feb 1989) ISSN: 8750-7587 [Print] United States
• PMID: 2540139 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antioxidants
• Hydroxides
• catalase-polyethylene glycol
• Superoxides
• Hydroxyl Radical
• Polyethylene Glycols
• Catalase
• Superoxide Dismutase
• polyethylene glycol-superoxide dismutase
• Glutathione
• Oxygen
• Glutathione Disulfide

Topics

• Animals
• Antioxidants (administration & dosage)
• Catalase (administration & dosage)
• Glutathione (analogs & derivatives, metabolism)
• Glutathione Disulfide
• Hydroxides (metabolism)
• Hydroxyl Radical
• Lung (drug effects, metabolism)
• Lung Injury
• Male
• Oxygen
• Polyethylene Glycols (administration & dosage)
• Pulmonary Edema (prevention & control)
• Rats
• Rats, Inbred Strains
• Superoxide Dismutase (administration & dosage)
• Superoxides (metabolism)