The effect of cathepsin K inhibitor on osteoclastic activity compared to alendronate and enamel matrix protein.

Abstract

BACKGROUND/AIM:

There have been several attempts to treat delayed replantation with agents that inhibit root resorption. The purpose of this study was to assess the effectiveness of cathepsin K inhibitor in inhibiting osteoclastic activity compared to that of alendronate and enamel matrix protein.

MATERIALS AND METHODS:

Murine RAW 264.7 cells were cultured in the presence of the receptor activator of NF-kB and lipopolysaccharide, followed by treatment with odanacatib, alendronate, or Emdogain at various concentrations. After drug treatment, an MTT assay was performed to evaluate cytotoxicity, while reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assays were performed to determine the expression levels of interleukin-1β, interleukin-6, prostaglandin E2, and tumor necrosis factor-α. Data were analyzed by one-way anova and Tukey's post-hoc test (P < 0.05).

RESULTS:

Of all tested agents, Emdogain resulted in the least cytotoxicity on RAW 264.7 cells, while 10(-9) M odanacatib had the largest suppressive effects on the expression levels of inflammatory cytokines.

CONCLUSIONS:

Odanacatib inhibits osteoclastic activity, showing the possibility as a treatment agent for delayed replantation of avulsed teeth.

• Authors: Wonkyung Yang, Hyunjung Ko, Heesun Kim, Miri Kim
• Journal: Dental traumatology : official publication of International Association for Dental Traumatology (Dent Traumatol) Vol. 31 Issue 3 Pg. 202-8 (Jun 2015) ISSN: 1600-9657 [Electronic] Denmark
• PMID: 25394885 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Biphenyl Compounds
• Dental Enamel Proteins
• Interleukin-1beta
• Interleukin-6
• Lipopolysaccharides
• RANK Ligand
• Tumor Necrosis Factor-alpha
• enamel matrix proteins
• Cathepsin K
• Dinoprostone
• odanacatib
• Alendronate

Topics

• Alendronate (pharmacology)
• Animals
• Biphenyl Compounds (pharmacology)
• Cathepsin K (antagonists & inhibitors)
• Cells, Cultured
• Dental Enamel Proteins (pharmacology)
• Dinoprostone (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Interleukin-1beta (metabolism)
• Interleukin-6 (metabolism)
• Lipopolysaccharides
• Mice
• Osteoclasts (drug effects)
• RANK Ligand (pharmacology)
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Necrosis Factor-alpha (metabolism)