Identification of hydrogen peroxide-secreting cells by cytocompatible coating with a hydrogel membrane.

Abstract

A method for identifying each cell secreting reactive oxygen species (ROS) is highly desirable to advance the understanding of the physiological and pathological processes attributed to extracellular ROS. Here, we first report a method for realizing this. The individual cells secreting hydrogen peroxide (H2O2), a common ROS, could be coated by a hydrogel membrane through a horseradish peroxidase-catalyzed reaction consuming H2O2 secreted from the cells themselves. This hydrogel membrane coating was proved to be cytocompatible. In addition, the hydrogel membrane made from an alginate derivative could be removed on demand without causing damage to the enclosed cells. These results demonstrated the feasibility of the proposed method to be an effective tool in cellular ROS studies.

• Authors: Yang Liu, Shinji Sakai, Shogo Kawa, Masahito Taya
• Journal: Analytical chemistry (Anal Chem) Vol. 86 Issue 23 Pg. 11592-8 (Dec 02 2014) ISSN: 1520-6882 [Electronic] United States
• PMID: 25359398 (Publication Type: Journal Article)

Chemical References

• Biocompatible Materials
• Hydrogel, Polyethylene Glycol Dimethacrylate
• Hydrogen Peroxide
• Horseradish Peroxidase

Topics

• Animals
• Biocatalysis
• Biocompatible Materials (chemistry)
• Cells (metabolism)
• Cells, Cultured
• Flow Cytometry
• Hep G2 Cells
• Horseradish Peroxidase (metabolism)
• Humans
• Hydrogel, Polyethylene Glycol Dimethacrylate (chemistry)
• Hydrogen Peroxide (analysis, metabolism)
• Mice