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Loss of expression of 5-hydroxymethylcytosine in CD30-positive cutaneous lymphoproliferative disorders.

AbstractBACKGROUND:
The methylation of DNA at position 5 of cytosine, and the subsequent reduction in intracellular 5-hydroxymethylcytosine (5-hmC) levels, is a key epigenetic event in several cancers, including systemic lymphomas. However, no studies have analyzed this epigenetic marker in cutaneous lymphomas. Therefore, we aimed to analyze the expression of 5-hmC in cutaneous CD30-positive lymphoproliferative disorders and compare it with a control group composed of reactive infectious and inflammatory disorders with CD30-positive cells.
METHODS:
Retrospective case series study with immunohistochemical analysis using anti-CD30 and anti-5-hmC antibodies in control (n = 19), lymphomatoid papulosis (LyP) (n = 27) and primary cutaneous anaplastic large cell lymphoma (ALCL) (n = 14) specimens.
RESULTS:
Complete loss of 5-hmC nuclear staining by CD30+ cells was observed in 63% of LyP cases, 57% of ALCL cases and 0% of control cases.
CONCLUSIONS:
The presence of 5-hmC+ and CD30+ lymphocytes was highly suggestive of a benign process. In contrast, loss of 5-hmC nuclear staining was highly suggestive of a lymphoproliferative disorder (ALCL or LyP). Under these circumstances, the use of 5-hmC staining can be a useful adjunctive tool for discriminating between neoplastic CD30+ lymphoproliferations and inflammatory/infectious simulators harboring reactive CD30+ cells.
AuthorsAieska De Souza, Marianne Tinguely, Madeleine Pfaltz, Daniel R Burghart, Werner Kempf
JournalJournal of cutaneous pathology (J Cutan Pathol) Vol. 41 Issue 12 Pg. 901-6 (Dec 2014) ISSN: 1600-0560 [Electronic] United States
PMID25353265 (Publication Type: Journal Article)
Copyright© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Chemical References
  • Biomarkers, Tumor
  • Ki-1 Antigen
  • 5-hydroxymethylcytosine
  • 5-Methylcytosine
  • Cytosine
Topics
  • 5-Methylcytosine (analogs & derivatives)
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor (genetics, metabolism)
  • Case-Control Studies
  • Child
  • Cytosine (analogs & derivatives, biosynthesis, metabolism)
  • Epigenesis, Genetic
  • Female
  • Humans
  • Immunohistochemistry (methods)
  • Ki-1 Antigen (metabolism)
  • Lymphoma, Primary Cutaneous Anaplastic Large Cell (genetics, metabolism, pathology)
  • Lymphomatoid Papulosis (metabolism, pathology)
  • Male
  • Middle Aged
  • Retrospective Studies
  • Skin Neoplasms (genetics, metabolism, pathology)
  • Young Adult

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