Abstract | BACKGROUND: The methylation of DNA at position 5 of cytosine, and the subsequent reduction in intracellular 5-hydroxymethylcytosine (5-hmC) levels, is a key epigenetic event in several cancers, including systemic lymphomas. However, no studies have analyzed this epigenetic marker in cutaneous lymphomas. Therefore, we aimed to analyze the expression of 5-hmC in cutaneous CD30-positive lymphoproliferative disorders and compare it with a control group composed of reactive infectious and inflammatory disorders with CD30-positive cells. METHODS: RESULTS: Complete loss of 5-hmC nuclear staining by CD30+ cells was observed in 63% of LyP cases, 57% of ALCL cases and 0% of control cases. CONCLUSIONS: The presence of 5-hmC+ and CD30+ lymphocytes was highly suggestive of a benign process. In contrast, loss of 5-hmC nuclear staining was highly suggestive of a lymphoproliferative disorder (ALCL or LyP). Under these circumstances, the use of 5-hmC staining can be a useful adjunctive tool for discriminating between neoplastic CD30+ lymphoproliferations and inflammatory/infectious simulators harboring reactive CD30+ cells.
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Authors | Aieska De Souza, Marianne Tinguely, Madeleine Pfaltz, Daniel R Burghart, Werner Kempf |
Journal | Journal of cutaneous pathology
(J Cutan Pathol)
Vol. 41
Issue 12
Pg. 901-6
(Dec 2014)
ISSN: 1600-0560 [Electronic] United States |
PMID | 25353265
(Publication Type: Journal Article)
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Copyright | © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
- Biomarkers, Tumor
- Ki-1 Antigen
- 5-hydroxymethylcytosine
- 5-Methylcytosine
- Cytosine
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Topics |
- 5-Methylcytosine
(analogs & derivatives)
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
(genetics, metabolism)
- Case-Control Studies
- Child
- Cytosine
(analogs & derivatives, biosynthesis, metabolism)
- Epigenesis, Genetic
- Female
- Humans
- Immunohistochemistry
(methods)
- Ki-1 Antigen
(metabolism)
- Lymphoma, Primary Cutaneous Anaplastic Large Cell
(genetics, metabolism, pathology)
- Lymphomatoid Papulosis
(metabolism, pathology)
- Male
- Middle Aged
- Retrospective Studies
- Skin Neoplasms
(genetics, metabolism, pathology)
- Young Adult
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