HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Purification of polysaccharides from Cordyceps militaris and their anti‑hypoxic effect.

Abstract
Acute mountain sickness, one of the most common altitude diseases, causes lung and brain injury. The present study aimed to investigate the anti‑hypoxic effect of purified polysaccharides extracted from Cordyceps militaris. The aqueous extract of Cordyceps militaris was purified progressively through a DEAE‑52 cellulose anion exchange column and a Sepharose G‑100 column. The fraction CMN1, with a molecular weight of 37842 Da, was the main fraction obtained and its chemical composition and structural characteristics were determined. CMN1 was found to have a monosaccharide composition of L‑rhamnose, L‑arabinose, D‑mannose, D‑galactose. The backbone of CMN1 comprised (1→2) and (1→3) linkages, with branched (1→6) and (1→4) linkages. The anti‑hypoxic effects of CMN1 were determined using a sodium nitrite toxicosis test, acute cerebral ischemic/hypoxic test and normobarie hypoxia test. CMN1 (0.5 g/kg) possessed a similar anti‑hypoxic effect to rhodiola oral liquid. Overall, the Cordyceps militaris polysaccharide, CMN1, was identified as an effective agent against hypoxia.
AuthorsYuan Dong, Shuang Hu, Chungang Liu, Qingfan Meng, Jia Song, Jiahui Lu, Yingkun Cheng, Chaohui Gao, Yan Liu, Di Wang, Lirong Teng
JournalMolecular medicine reports (Mol Med Rep) Vol. 11 Issue 2 Pg. 1312-7 (Feb 2015) ISSN: 1791-3004 [Electronic] Greece
PMID25351532 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Polysaccharides
  • Sodium Nitrite
Topics
  • Animals
  • Apoptosis (drug effects)
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Cordyceps (metabolism)
  • Female
  • Hypoxia
  • Male
  • Methemoglobinemia (chemically induced, drug therapy, mortality)
  • Mice
  • Molecular Weight
  • Polysaccharides (isolation & purification, pharmacology, therapeutic use)
  • Sodium Nitrite (toxicity)
  • Spectroscopy, Fourier Transform Infrared
  • Survival Rate

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: