Expression of CDC25A and CDC25B phosphatase proteins in human retinoblastoma and its correlation with clinicopathological parameters.

Abstract

BACKGROUND:

CDC25 proteins play a pivotal role in controlling cell proliferation during development and tumorigenesis. The aim of the study is to elucidate the role of CDC25A and CDC25B proteins in retinoblastoma and their association with the clinical and histopathological parameters.

METHODS:

One hundred and nine prospective cases of primary enucleated retinoblastomas were included in the present study. Expression of CDC25A and CDC25B proteins was investigated by immunohistochemistry, western blotting and mRNA expression by reverse-transcriptase PCR.

RESULTS:

Immunohistochemistry showed CDC25A expression in (57/109) 52.29%, whereas CDC25B expressed in (69/109) 63.30% cases. Western blotting confirmed the immunoreactivity results on representative cases. mRNA expression of CDC25A and CDC25B was found in 29/60 (48.33%) and 35/60 (58.33%) cases, respectively. Expression of CDC25A and CDC25B showed significant correlation with poor tumour differentiation and tumour invasion (p<0.05). There was a statistically significant difference in the overall survival of patients with CDC25B expression (p=0.0270).

CONCLUSIONS:

Our results suggest that expression of CDC25B may be used as a potential prognostic marker in the pathogenesis of retinoblastoma. These findings demonstrate an important role of CDC25 phosphatase proteins and inhibition of these proteins may have therapeutic potential in retinoblastoma.

• Authors: Lata Singh, Neelam Pushker, Seema Sen, Mithalesh Kumar Singh, Sameer Bakhshi, Bhavna Chawla, Seema Kashyap
• Journal: The British journal of ophthalmology (Br J Ophthalmol) Vol. 99 Issue 4 Pg. 457-63 (Apr 2015) ISSN: 1468-2079 [Electronic] England
• PMID: 25326518 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Chemical References

• Biomarkers, Tumor
• RNA, Messenger
• RNA, Neoplasm
• CDC25A protein, human
• CDC25B protein, human
• cdc25 Phosphatases

Topics

• Biomarkers, Tumor (genetics, metabolism)
• Blotting, Western
• Child
• Child, Preschool
• Eye Enucleation
• Female
• Humans
• Immunohistochemistry
• Infant
• Male
• Prospective Studies
• RNA, Messenger (genetics)
• RNA, Neoplasm (genetics)
• Retinal Neoplasms (enzymology, pathology)
• Retinoblastoma (enzymology, pathology)
• Reverse Transcriptase Polymerase Chain Reaction
• cdc25 Phosphatases (genetics, metabolism)