The stability of hexacosanoyl lysophosphatidylcholine in dried-blood spot quality control materials for X-linked adrenoleukodystrophy newborn screening.
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| Abstract | OBJECTIVES: Newborn screening for X-linked adrenoleukodystrophy utilizes tandem mass spectrometry to analyze dried-blood spot specimens. Quality control materials (dried-blood spots enriched with hexacosanoyl lysophosphatidylcholine) were prepared and stored at different temperatures for up to 518days to evaluate the stability of this biomarker for X-linked adrenoleukodystrophy. DESIGN AND METHODS: Dried-blood spot storage included desiccant (45, 171, and 518days) or omitted desiccant (53days at >90% relative humidity). Specimens were stored for 171 and 518days at -20°C, 4°C, ambient temperature, and 37°C. Each weekday for 45days, a bag of specimens stored at 4°C was warmed to ambient temperature and one specimen was removed for storage at -80°C. Specimens were analyzed by high-performance liquid-chromatography electrospray ionization tandem mass spectrometry and data was plotted as concentration (micromoles per liter) vs. time. Linear regression provided slope and y-intercept values for each storage condition. RESULTS: Small slope values (0.01 or less) and y-intercept values close to the enrichment indicated less than 11% loss of hexacosanoyl lysophosphatidylcholine under all storage conditions tested. CONCLUSIONS: Quality control materials for X-linked adrenoleukodystrophy are stable for at least 1year when stored with desiccant.
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| Authors | Christopher A Haynes, Víctor R De Jesús |
| Journal | Clinical biochemistry (Clin Biochem) Vol. 48 Issue 1-2 Pg. 8-10 (Jan 2015) ISSN: 1873-2933 [Electronic] United States |
| PMID | 25307302 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.) |
| Copyright | Published by Elsevier Inc. |
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