Ultraselective electrochemiluminescence biosensor based on locked nucleic acid modified toehold-mediated strand displacement reaction and junction-probe.

Abstract

Locked nucleic acid (LNA) is applied in toehold-mediated strand displacement reaction (TMSDR) to develop a junction-probe electrochemiluminescence (ECL) biosensor for single-nucleotide polymorphism (SNP) detection in the BRCA1 gene related to breast cancer. More than 65-fold signal difference can be observed with perfectly matched target sequence to single-base mismatched sequence under the same conditions, indicating good selectivity of the ECL biosensor.

• Authors: Xi Zhang, Jing Zhang, Dongzhi Wu, Zhijing Liu, Shuxian Cai, Mei Chen, Yanping Zhao, Chunyan Li, Huanghao Yang, Jinghua Chen
• Journal: The Analyst (Analyst) Vol. 139 Issue 23 Pg. 6109-12 (Dec 07 2014) ISSN: 1364-5528 [Electronic] England
• PMID: 25299420 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BRCA1 Protein
• BRCA1 protein, human
• Oligonucleotides
• locked nucleic acid
• DNA

Topics

• BRCA1 Protein (isolation & purification)
• Base Sequence
• Biosensing Techniques (instrumentation, methods)
• DNA (genetics)
• Electrochemical Techniques (instrumentation, methods)
• Humans
• Luminescence
• Oligonucleotides (chemistry)
• Point Mutation