Abstract | PURPOSE: METHODS: AtT-20 pituitary corticotroph tumor cells were cultured. The expression levels of mouse proopiomelanocortin ( POMC) and pituitary tumor transforming gene 1 (PTTG1) mRNA were evaluated using quantitative real-time PCR. Cellular DNA content was analyzed with fluorescence-activated cell sorting (FACS) analysis. The protein levels were determined by Western blot analysis. RESULTS: Both 17-allylamino-17-demethoxygeldanamycin and CCT018159 decreased POMC mRNA levels in AtT-20 cells and ACTH levels in the culture medium of these cells, suggesting that both drugs suppress ACTH synthesis and secretion in corticotroph tumor cells. Both drugs also decreased cell proliferation and induced apoptosis. FACS analyses revealed that both agents increased the percentage of AtT-20 cells in the G2/M phase. These drugs decreased cell proliferation, presumably due to the induction of cell death and arrest of the cell cycle in AtT-20 cells. Tumor weight in mice xenografted with AtT-20 cells and treated with CCT018159 was lower than in AtT-20-xenografted control mice. CCT018159 also decreased plasma ACTH levels, and POMC and PTTG1 mRNA levels in the tumor cells. CONCLUSIONS:
CCT018159 inhibits ACTH production and corticotroph tumor cell proliferation in vitro and in vivo.
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Authors | Aya Sugiyama, Kazunori Kageyama, Shingo Murasawa, Noriko Ishigame, Kanako Niioka, Makoto Daimon |
Journal | Pituitary
(Pituitary)
Vol. 18
Issue 4
Pg. 542-53
(Aug 2015)
ISSN: 1573-7403 [Electronic] United States |
PMID | 25280813
(Publication Type: Journal Article)
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Chemical References |
- Benzoquinones
- CCT018159
- HSP90 Heat-Shock Proteins
- Heterocyclic Compounds, 2-Ring
- Lactams, Macrocyclic
- PTTG1 protein, mouse
- Pyrazoles
- RNA, Messenger
- Securin
- tanespimycin
- Pro-Opiomelanocortin
- Adrenocorticotropic Hormone
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Topics |
- ACTH-Secreting Pituitary Adenoma
(genetics, metabolism, pathology)
- Adenoma
(genetics, metabolism, pathology)
- Adrenocorticotropic Hormone
(drug effects, metabolism)
- Animals
- Benzoquinones
(pharmacology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- HSP90 Heat-Shock Proteins
(antagonists & inhibitors)
- Heterocyclic Compounds, 2-Ring
(pharmacology)
- Lactams, Macrocyclic
(pharmacology)
- Mice
- Neoplasm Transplantation
- Pro-Opiomelanocortin
(drug effects, genetics)
- Pyrazoles
(pharmacology)
- RNA, Messenger
(drug effects, metabolism)
- Real-Time Polymerase Chain Reaction
- Securin
(drug effects, genetics)
- Tumor Burden
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