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High similarity of Trypanosoma cruzi kDNA genetic profiles detected by LSSP-PCR within family groups in an endemic area of Chagas disease in Brazil.

AbstractINTRODUCTION:
Determining the genetic similarities among Trypanosoma cruzi populations isolated from different hosts and vectors is very important to clarify the epidemiology of Chagas disease.
METHODS:
An epidemiological study was conducted in a Brazilian endemic area for Chagas disease, including 76 chronic chagasic individuals (96.1% with an indeterminate form; 46.1% with positive hemoculture).
RESULTS:
T. cruzi I (TcI) was isolated from one child and TcII was found in the remaining (97.1%) subjects. Low-stringency single-specific-primer-polymerase chain reaction (LSSP-PCR) showed high heterogeneity among TcII populations (46% of shared bands); however, high similarities (80-100%) among pairs of mothers/children, siblings, or cousins were detected.
CONCLUSIONS:
LSSP-PCR showed potential for identifying similar parasite populations among individuals with close kinship in epidemiological studies of Chagas disease.
AuthorsSandra Maria Alkmim-Oliveira, Henrique Borges Kappel, Cristiane Pontes Andrade, Aluízio Prata, Luis Eduardo Ramirez, Dalmo Correia, Eliane Lages-Silva
JournalRevista da Sociedade Brasileira de Medicina Tropical (Rev Soc Bras Med Trop) 2014 Sep-Oct Vol. 47 Issue 5 Pg. 653-6 ISSN: 1678-9849 [Electronic] Brazil
PMID25271788 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA, Kinetoplast
  • DNA, Protozoan
Topics
  • Adolescent
  • Adult
  • Brazil (epidemiology)
  • Chagas Disease (epidemiology, parasitology)
  • Child
  • Child, Preschool
  • DNA, Kinetoplast (genetics)
  • DNA, Protozoan
  • Female
  • Genetic Variation
  • Genotype
  • Humans
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Trypanosoma cruzi (genetics, isolation & purification)
  • Young Adult

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