[Dachengqi decoction reduces the serum levels of mast cell tryptase and inflammatory cytokines in rabbits with post-cardiac arrest syndrome].

Abstract

OBJECTIVE:

To observe the effects of Dachengqi decoction on serum levels of mast cell tryptase, monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) in rabbits with post-cardiac arrest syndrome (PCAS).

METHODS:

Thirty healthy male Japanese rabbits were randomly divided into three groups: sham-operation group, PCAS model group and Dachengqi decoction treatment group. The model of PCAS was established by asphyxia-induced cardiac arrest. Fifteen minutes after return of spontaneous circulation, Dachengqi decoction [15 g/(kg.d), bid] was given by intra-gastric administration in Dachengqi decoction treatment group. The indicators of organ function were evaluated 24, 48 and 72 hours after cardiac arrest. The serum levels of mast cell tryptase, MCP-1 and IL-8 were determined by ELISA.

RESULTS:

Dachengqi decoction alleviated the dysfunction significantly in heart, brain, liver and kidney. Compared with the sham group, the serum levels of mast cell tryptase, MCP-1 and IL-8 increased significantly in PCAS group (P<0.01). Compared with the PCAS group, the serum levels of mast cell tryptase (at 6 hours), MCP-1 (at 6, 24 and 48 hours) and IL-8 (at 6 and 24 hours) decreased significantly in Dachengqi decoction treatment group (P<0.05 or P<0.01).

CONCLUSION:

Dachengqi decoction can reduce the serum levels of mast cell tryptase, MCP-1 and IL-8 in rabbits with post-cardiac arrest syndrome.

• Authors: Jingning Yang, Min Xiao, Xuejun Wang, Xiaoyan Li, Pengfei Liang, Lin Chai, Ming Luo
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 30 Issue 10 Pg. 1026-9 (Oct 2014) ISSN: 1007-8738 [Print] China
• PMID: 25270202 (Publication Type: English Abstract, Journal Article)

Chemical References

• Chemokine CCL2
• Interleukin-8
• Plant Extracts
• dachengqi decoction
• Tryptases

Topics

• Animals
• Chemokine CCL2 (blood)
• Enzyme-Linked Immunosorbent Assay
• Heart Arrest (blood, physiopathology, prevention & control)
• Interleukin-8 (blood)
• Male
• Phytotherapy
• Plant Extracts (pharmacology)
• Rabbits
• Random Allocation
• Syndrome
• Treatment Outcome
• Tryptases (blood)