Abstract | BACKGROUND: METHODS: Plasma samples were isolated from 71 ITP patients and 136 non- ITP controls and incubated with platelets from normal individuals. After washing, platelets were lysed and the platelet lysates were incubated with polystyrene microbeads coupled with monoclonal antibodies against human GPs IX (SZ1), Ib (SZ2), IIIa (SZ21), IIb (SZ22), and P-selectin (SZ51). Platelet GP- autoantibody complexes were detected by flow cytometry using a FITC-labeled secondary antibody. RESULT:
Autoantibodies against platelet GPIb, GPIIb, GPIIIa, GPIX and P-selectin were detected in plasma from ITP patients, as indicated by high mean fluorescent intensity values when microbeads with antibodies SZ1, SZ2, SZ21, SZ22, and SZ51 were used. In ROC analysis, values of the area under the curve were 0.74, 0.83, 0.80, 0.79 and 0.87, respectively. Compared with the previously reported assays, this new FCIA eliminated the need of isolating platelets from ITP patients without compromising assay sensitivity and accuracy in predicting ITP. CONCLUSION: This simplified FICA assay may be more suitable for ITP diagnosis in clinical laboratory settings.
|
Authors | Yunxiao Zhao, Mingqing Zhu, Miao Jiang, Bin Zuo, Qingyu Wu, Changgeng Ruan, Yang He |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 438
Pg. 396-400
(Jan 01 2015)
ISSN: 1873-3492 [Electronic] Netherlands |
PMID | 25269089
(Publication Type: Journal Article)
|
Copyright | Copyright © 2014 Elsevier B.V. All rights reserved. |
Chemical References |
- Antibodies, Monoclonal
- Autoantibodies
- Platelet Membrane Glycoproteins
- Polystyrenes
- Fluorescein-5-isothiocyanate
|
Topics |
- Adolescent
- Adult
- Aged
- Antibodies, Monoclonal
- Area Under Curve
- Autoantibodies
(blood)
- Blood Platelets
(chemistry, immunology)
- Case-Control Studies
- Child
- Child, Preschool
- Female
- Flow Cytometry
(instrumentation, standards)
- Fluorescein-5-isothiocyanate
- Humans
- Immunoassay
(instrumentation, standards)
- Male
- Microspheres
- Middle Aged
- Platelet Membrane Glycoproteins
(analysis, immunology)
- Polystyrenes
- Protein Array Analysis
(instrumentation, standards)
- Purpura, Thrombocytopenic, Idiopathic
(blood, diagnosis, immunology)
- ROC Curve
- Staining and Labeling
(methods)
|