Pallidifloside D, a saponin glycoside constituent from Smilax riparia, resist to hyperuricemia based on URAT1 and GLUT9 in hyperuricemic mice.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

The roots and rhizomes of Smilax riparia (SR), called "Niu-Wei-Cai" in traditional Chinese medicine (TCM), are believed to be effective in treating hyperuricemia and gout symptoms. This study was designed to isolate a saponin glycoside named pallidifloside D from the total saponins of Smilax riparia and to examine its effect in reducing serum uric acid levels in a hyperuricemic mouse model induced by potassium oxonate.

MATERIALS AND METHODS:

We examined the effects of pallidifloside D treated with 5, 10 and 20mg/kg on serum uric acid levels (SUA), Serum creatinine (SCr) and blood urea nitrogen (BUN) levels in a hyperuricemic mouse. A colorimetric method was used to evaluate the effects of pallidifloside D on the XOD activities, and Western Blotting analysis were carried out to observe protein levels of mURAT1, mGLUT9 and mOTA1 in hyperuricemic mice after treatment with pallidifloside D.

RESULTS:

The levels of serum uric acid levels (SUA) were suppressed significantly with dose-dependence by pallidifloside D treated with 5, 10 and 20mg/kg (p<0.05, p<0.01 and p<0.01 respectively). Pallidifloside D could down-regulate the expression levels of renal mURAT1 protein in hyperuricemic mice in a dose-dependent manner (p<0.05, p<0.01, and p<0.001 respectively), and the protein levels of mGLUT9 could be down-regulated with dose-dependence (p<0.05 and p<0.01 respectively) by pallidifloside D at the dose of 10 and 20mg/kg.

CONCLUSION:

These results suggest that pallidifloside D possesses a potent uricosuric effect in hyperuricemic mice through decreasing renal mURAT1 and GLUT9, which contribute to the enhancement of uric acid excretion and attenuate hyperuricemia-induced renal dysfunction.

• Authors: Xiao-Hui Wu, Jin-Lan Ruan, Jun Zhang, Shu-Qing Wang, Yan-Wen Zhang
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 157 Pg. 201-5 (Nov 18 2014) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 25267580 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Glucose Transport Proteins, Facilitative
• Organic Anion Transporters
• Saponins
• Slc22a12 protein, mouse
• Slc2a9 protein, mouse
• pallidifloside D
• Uric Acid
• potassium oxonate
• Oxonic Acid

Topics

• Animals
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Down-Regulation (drug effects)
• Glucose Transport Proteins, Facilitative (genetics)
• Hyperuricemia (drug therapy)
• Male
• Medicine, Chinese Traditional
• Mice
• Organic Anion Transporters (genetics)
• Oxonic Acid (toxicity)
• Plant Roots
• Rhizome
• Saponins (administration & dosage, isolation & purification, pharmacology)
• Smilax (chemistry)
• Uric Acid (blood)