GADD45γ induces G2/M arrest in human pharynx and nasopharyngeal carcinoma cells by cucurbitacin E.

Abstract

Nasopharyngeal carcinoma (NPC) is a common form of malignant cancer, for which radiotherapy or chemotherapy are the main treatment methods. Cucurbitacin E (CuE) is a natural compound-based drug which from the climbing stem of Cucumic melo L (Guadi). Previously shown to be an antifeedant as well as a potent chemopreventive agent against several types of cancer. The present study, investigated anti-proliferation and cell cycle G2/M arrest induced by CuE in Detroit 562 cells (pharynx carcinoma) and HONE-1 (nasopharyngeal carcinoma) cells. Results indicate that the cytotoxicity is associated with accumulation in G2/M cell-cycle phases. CuE produced cell cycle arrest as well as the downregulation of cyclin B1 and CDC2 expression. In addition, treated cells with CuE and GADD45γ SiRNA that also coincided with GADD45γ gene activation in cell cycle arrest. Both effects increased proportionally with the dose of CuE; however, proliferation inhibition and mitosis delay was dependant on the amount of CuE treatment in the cancer cells.

• Authors: Chao-Ming Hung, Chi-Chang Chang, Chen-Wei Lin, Chih-Chen Chen, Yi-Chiang Hsu
• Journal: Scientific reports (Sci Rep) Vol. 4 Pg. 6454 (Sep 23 2014) ISSN: 2045-2322 [Electronic] England
• PMID: 25245461 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• GADD45 protein
• Intracellular Signaling Peptides and Proteins
• RNA, Messenger
• RNA, Small Interfering
• Triterpenes
• cucurbitacin E

Topics

• Apoptosis (drug effects)
• Blotting, Western
• Carcinoma
• Cell Cycle Checkpoints (drug effects)
• Cell Division (drug effects)
• Cell Proliferation (drug effects)
• G2 Phase (drug effects)
• Humans
• Immunoprecipitation
• Intracellular Signaling Peptides and Proteins (antagonists & inhibitors, genetics, metabolism)
• Mitosis (drug effects)
• Nasopharyngeal Carcinoma
• Nasopharyngeal Neoplasms (drug therapy, metabolism, pathology)
• Pharynx (drug effects, metabolism)
• RNA, Messenger (genetics)
• RNA, Small Interfering (genetics)
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Triterpenes (pharmacology)
• Tumor Cells, Cultured