16S rRNA gene sequencing, multilocus sequence analysis, and mass spectrometry identification of the proposed new species "Clostridium neonatale".

Abstract

In 2002, an outbreak of necrotizing enterocolitis in a Canadian neonatal intensive care unit was associated with a proposed novel species of Clostridium, "Clostridium neonatale." To date, there are no data about the isolation, identification, or clinical significance of this species. Additionally, C. neonatale has not been formally classified as a new species, rendering its identification challenging. Indeed, the C. neonatale 16S rRNA gene sequence shows high similarity to another Clostridium species involved in neonatal necrotizing enterocolitis, Clostridium butyricum. By performing a polyphasic study combining phylogenetic analysis (16S rRNA gene sequencing and multilocus sequence analysis) and phenotypic characterization with mass spectrometry, we demonstrated that C. neonatale is a new species within the Clostridium genus sensu stricto, for which we propose the name Clostridium neonatale sp. nov. Now that the status of C. neonatale has been clarified, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) can be used for better differential identification of C. neonatale and C. butyricum clinical isolates. This is necessary to precisely define the role and clinical significance of C. neonatale, a species that may have been misidentified and underrepresented during previous neonatal necrotizing enterocolitis studies.

• Authors: Philippe Bouvet, Laurent Ferraris, Brunhilde Dauphin, Michel-Robert Popoff, Marie Jose Butel, Julio Aires
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 52 Issue 12 Pg. 4129-36 (Dec 2014) ISSN: 1098-660X [Electronic] United States
• PMID: 25232167 (Publication Type: Journal Article)
• Copyright: Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Chemical References

• DNA, Bacterial
• DNA, Ribosomal
• RNA, Ribosomal, 16S

Topics

• Canada (epidemiology)
• Clostridium (chemistry, classification, genetics, isolation & purification)
• Cluster Analysis
• DNA, Bacterial (chemistry, genetics)
• DNA, Ribosomal (chemistry, genetics)
• Disease Outbreaks
• Enterocolitis, Necrotizing (epidemiology, microbiology)
• Genes, rRNA
• Humans
• Infant, Newborn
• Intensive Care Units, Neonatal
• Molecular Sequence Data
• Multilocus Sequence Typing
• Phylogeny
• RNA, Ribosomal, 16S (genetics)
• Sequence Analysis, DNA
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization