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Idiotypic intramolecular help. Induction of tumor-specific antibodies by monoclonal anti-idiotypic antibody with the help of Fc-specific T helper clones.

Abstract
In this study, the mechanism of anti-Id vaccination was investigated by using cloned Th cells and an anti-idiotypic mAb. 2F10, an anti-idiotypic mAb derived from an Igh1-e allotype mouse strain, which induces protection against the L1210/GZL DBA/2 tumor, was used to prime DBA/2 mice. An Fc (Igh1-e)-specific syngeneic Th clone was cocultured in the presence of 2F10 anti-Id with 2F10-Fab-primed B cells. The Th clone responded with proliferation and also provided help for 2F10-Fab-primed B cells to produce antibodies that bind to L1210/GZL and not to P815 tumor cells. Intact 2F10 anti-Id was presented to Fc-specific Th cells by Fab (or Id) primed B cells more efficiently than the fragment mixture (Fab plus Fc) of 2F10 anti-Id, indicating that 2F10-Fab (or Id)-primed B cells capture 2F10 anti-Id through surface Ig receptors. Presenting B cells are sensitive to treatment with chloroquine and must come from H-2 matched mice, indicating that the Ag presentation by Fab-primed B cells to Fc-specific Th cells requires processing and is MHC restricted. Collectively these results outline a mechanism that may operate in anti-Id therapy of tumor-bearing animals by using tumor Ag mimicking anti-idiotypic antibodies. A similar mechanism could be effective in tumor patients immunized with xenogeneic anti-idiotypic antibodies operating under the "intra(Ag) molecular help."
AuthorsY Saeki, J J Chen, L F Shi, H Kohler
JournalJournal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 142 Issue 8 Pg. 2629-34 (Apr 15 1989) ISSN: 0022-1767 [Print] United States
PMID2522964 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Antibodies, Monoclonal
  • Antibodies, Neoplasm
  • Antigens, Neoplasm
  • Immunoglobulin Fc Fragments
  • Immunoglobulin Idiotypes
  • Vaccines
Topics
  • Animals
  • Antibodies, Monoclonal (immunology)
  • Antibodies, Neoplasm (biosynthesis)
  • Antibody Specificity
  • Antigens, Neoplasm (immunology)
  • B-Lymphocytes (immunology)
  • Immunoglobulin Fc Fragments (immunology)
  • Immunoglobulin Idiotypes (immunology)
  • Major Histocompatibility Complex
  • Mice
  • Mice, Inbred Strains
  • T-Lymphocytes, Helper-Inducer (immunology)
  • Vaccines (immunology)

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