The value of anti-desmoglein enzyme-linked immunosorbent assay in the immunological follow-up of pemphigus.

Abstract

BACKGROUND:

The value of anti-desmoglein 1 and 3 (Dsg1, Dsg3) enzyme-linked immunosorbent assay (ELISA) is controversial in the follow-up of pemphigus.

OBJECTIVE:

To evaluate anti-desmoglein ELISA (Dsg ELISA) in the follow-up of pemphigus and compare ELISA with direct and indirect immunofluorescence in complete remission (CR).

METHODS:

We performed a retrospective monocenter study of patients with pemphigus and consecutive sera samples collected at baseline (M0), 12 months (M12) and 24 or 36 months after M0 (M24/36). Tests were compared in CR and in active disease. Direct immunofluorescence and circulating autoantibodies were compared for patients with stable CR.

RESULTS:

We included 36 patients. At M12, ELISA values did not differ between CR and active disease. At M24/36, Dsg3 but not Dsg1 ELISA values were lower in CR (p = 0.07). For 5/8 patients with stable CR, direct immunofluorescence and ELISA findings remained positive.

CONCLUSION:

In routine practice, Dsg ELISA seems to be of little interest for immunological follow-up of pemphigus.

• Authors: Nathalia Bellon, Chantal André, Emilie Sbidian, Nicolas Ortonne, Pierre Wolkenstein, Olivier Chosidow, Saskia Ingen-Housz-Oro
• Journal: Dermatology (Basel, Switzerland) (Dermatology) Vol. 229 Issue 3 Pg. 256-62 ( 2014) ISSN: 1421-9832 [Electronic] Switzerland
• PMID: 25227321 (Publication Type: Comparative Study, Evaluation Study, Journal Article)

Chemical References

• Desmoglein 1
• Desmoglein 3

Topics

• Adult
• Aged
• Databases, Factual
• Desmoglein 1 (analysis, blood)
• Desmoglein 3 (blood)
• Disease Progression
• Enzyme-Linked Immunosorbent Assay (methods)
• Female
• Fluorescent Antibody Technique, Direct (methods)
• Fluorescent Antibody Technique, Indirect (methods)
• Follow-Up Studies
• France
• Humans
• Male
• Middle Aged
• Monitoring, Physiologic (methods)
• Pemphigus (blood, physiopathology)
• Retrospective Studies
• Sensitivity and Specificity
• Severity of Illness Index
• Statistics, Nonparametric
• Young Adult