PET and NIR optical imaging using self-illuminating (64)Cu-doped chelator-free gold nanoclusters.
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| Abstract |
Self-illuminating fluorescence imaging without autofluorescence background interference has recently aroused more research interests in molecular imaging. Currently, only a few self-illuminating probes were developed, based mainly on toxic quantum dots such as CdSe, CdTe. Herein, we report a novel design of nontoxic self-illuminating gold nanocluster ((64)Cu-doped AuNCs) for dual-modality positron emission tomography (PET) and near-infrared (NIR) fluorescence imaging based on Cerenkov resonance energy transfer (CRET). PET radionuclide (64)Cu was introduced by a chelator-free doping method, which played dual roles as the energy donor and the PET imaging source. Meanwhile, AuNCs acted as the energy acceptor for NIR fluorescence imaging. (64)Cu-doped AuNCs exhibited efficient CRET-NIR and PET imaging both in vitro and in vivo. In a U87MG glioblastoma xenograft model, (64)Cu-doped AuNCs showed high tumor uptake (14.9 %ID/g at 18 h) and produced satisfactory tumor self-illuminating NIR images in the absence of external excitation. This self-illuminating nanocluster with non-toxicity and good biocompatibility can be employed as a novel imaging contrast agent for biomedical applications, especially for molecular imaging.
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| Authors | Hao Hu, Peng Huang, Orit Jacobson Weiss, Xuefeng Yan, Xuyi Yue, Molly Gu Zhang, Yuxia Tang, Liming Nie, Ying Ma, Gang Niu, Kaichun Wu, Xiaoyuan Chen |
| Journal | Biomaterials (Biomaterials) Vol. 35 Issue 37 Pg. 9868-9876 (Dec 2014) ISSN: 1878-5905 [Electronic] Netherlands |
| PMID | 25224367 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't) |
| Copyright | Published by Elsevier Ltd. |
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