Interleukin-6 (IL-6)-597 A/G (rs1800797) & -174 G/C (rs1800795) gene polymorphisms in type 2 diabetes.

Abstract

BACKGROUND & OBJECTIVES:

Diabetes is a metabolic pro-inflammatory disorder characterized by chronic hyperglycaemia and increased levels of circulating cytokines suggesting a causal role for inflammation in its aetiology. In order to decipher the role of interleukin-6 (IL-6) in type 2 diabetes mellitus (T2DM) we analyzed two promoter polymorphisms -597 A/G (rs1800797) and -174 G/C (rs1800795) in T2DM cases from north India, and in healthy controls.

METHODS:

DNA was isolated from venous blood samples of T2DM patients (n=213) and normal healthy controls (n=145). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed after biochemical analysis. The genotypic and allelic frequency distributions were analyzed.

RESULTS:

The clinical/biochemical parameters of T2DM cases when compared to controls showed a significant difference. No significant association was observed with -597A/G polymorphism while, -174 G/C showed a highly significant association (P<0.001). In haplotypic analysis, combination of -597GFNx01/-174CFNx01 showed significant association (P=0.010).

INTERPRETATION & CONCLUSIONS:

Our data suggest that IL-6 gene polymorphisms play a prominent role in T2DM disease susceptibility in population from north India.

• Authors: Madhukar Saxena, C G Agrawal, Neena Srivastava, Monisha Banerjee
• Journal: The Indian journal of medical research (Indian J Med Res) Vol. 140 Issue 1 Pg. 60-8 (Jul 2014) ISSN: 0971-5916 [Print] India
• PMID: 25222779 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-6

Topics

• Adult
• Aged
• Base Sequence
• Diabetes Mellitus, Type 2 (genetics)
• Female
• Gene Components
• Gene Frequency
• Genetic Predisposition to Disease (genetics)
• Genotype
• Haplotypes (genetics)
• Humans
• India
• Interleukin-6 (genetics)
• Male
• Middle Aged
• Molecular Sequence Data
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Polymorphism, Single Nucleotide (genetics)
• Promoter Regions, Genetic (genetics)