[Research on expression and function of phosphorylated DARPP-32 on pentylenetetrazol-induced epilepsy model of rat].

Abstract

The present study is to explore the change process and distribution of phosphorylated DARPP-32 (p-DARPP-32) in rat brain including cortex, hippocampus and striatum and to further deduce whether p-DARPP-32 was possibly involved in epilepsy induced by repetitive low doses of pentylenetetrazol (PTZ). PTZ-induced epilepsy model in rat was established with 30 male SD rats randomly divided into 6 groups, control group and five trial groups [PTZ 1 h, PTZ 6 h, PTZ 24 h, PTZ 48 h and PTZ 72 h respectively, after onset of status epilepticus (SE)]. Immunohistochemistry and immunofluorescence double-labeling were used to detect the temporal time change and distribution of p-DARPP-32 expression and to analyze the coexpression of DARPP-32 and p-DARPP-32 in rat brain after the onset of PTZ-induced generalized SE. The results showed that there was a temporal time change of p-DARPP-32 expression in rat brain after the onset of SE. The number of p-DARPP-32-positive cells increased significantly and reached the peaks at the ends of 1 hour and 6 hours after the onset of SE, but decreased at the end of 24 hours. The moderate to strong p-DARPP-32-immunopositive neurons were observed in cortex, hippocampus and striatum, and located in cell cytoplasm and cell nucleus. Further immunofluorescence double-labeling revealed that denser colocalization of p-DARPP-32 and DARPP-32 in the neurons existed in the area mentioned above. Therefore, PTZ-induced SE may cause phosphorylation of DARPP-32 in rat brain. The temporal time change and distribution of p-DARPP-32 suggest that phosphorylation of DARPP-32 may be involved in PTZ-induced epilepsy in rat brain including cortex, hippocampus and striatum, and p-DARPP-32 may play a central role in the onset of SE.

• Authors: Weiwen Wang, Xiaoyang Liao, Zhenghui Yang, Hang Lin, Qingsong Wang, Yuxian Wu, Yu Liu
• Journal: Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi (Sheng Wu Yi Xue Gong Cheng Xue Za Zhi) Vol. 31 Issue 3 Pg. 637-41 (Jun 2014) ISSN: 1001-5515 [Print] China
• PMID: 25219249 (Publication Type: Journal Article)

Chemical References

• Dopamine and cAMP-Regulated Phosphoprotein 32
• Ppp1r1b protein, rat
• Pentylenetetrazole

Topics

• Animals
• Cerebral Cortex (metabolism)
• Corpus Striatum (metabolism)
• Dopamine and cAMP-Regulated Phosphoprotein 32 (metabolism)
• Hippocampus (metabolism)
• Male
• Neurons (metabolism)
• Pentylenetetrazole
• Rats
• Rats, Sprague-Dawley
• Status Epilepticus (chemically induced, metabolism)