Inhibition of experimental metastasis of murine fibrosarcoma cells by oligopeptide analogues to the fibronectin cell-binding site.

We have analyzed the effect of the synthetic oligopeptides Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP) and Gly-Arg-Gly-Glu-Ser-Pro (GRGESP), analogues to the fibronectin cell-binding sequence, on the formation of experimental lung metastasis. SR-BALB were injected alone or in conjunction with GRGDSP or GRGESP in the tail vein of BALB/c mice. Co-injection with GRGESP reduced by approximately 70% the number of metastatic colonies per mouse. However, co-injection with the closely related peptide GRGDSP, containing the conservative substitution Glu/Asp, did not affect metastatic behavior. GRGESP peptide anti-metastatic activity was not due to a toxic effect on tumor cells or on mice. In vitro adhesion assays testing for a possible effect of the peptide on cell-matrix interactions indicated that the GRGESP peptide did not affect cell adhesion to the matrix proteins tested.
AuthorsS Bretti, P Neri, L Lozzi, M Rustici, P Comoglio, F Giancotti, G Tarone
JournalInternational journal of cancer. Journal international du cancer (Int J Cancer) Vol. 43 Issue 1 Pg. 102-6 (Jan 15 1989) ISSN: 0020-7136 [Print] UNITED STATES
PMID2521334 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Oligopeptides
  • Receptors, Fibronectin
  • Receptors, Immunologic
  • glycyl-arginyl-glycyl-aspartyl-seryl-proline
  • glycyl-arginyl-glycyl-glutamyl-seryl-proline
  • Animals
  • Cell Adhesion
  • Cell Line
  • Enzyme-Linked Immunosorbent Assay
  • Fibrosarcoma (pathology)
  • Lung Neoplasms (secondary)
  • Mice
  • Mice, Inbred BALB C
  • Neoplasm Metastasis (prevention & control)
  • Neoplasm Transplantation
  • Oligopeptides (pharmacology)
  • Receptors, Fibronectin
  • Receptors, Immunologic (analysis)

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