Abstract | OBJECTIVE: APPROACH AND RESULTS: SR-BI/LDLR double knockout and control LDLR knockout mice were fed atherogenic diets containing different amounts of fat, cholesterol, and sodium cholate. Double knockout mice fed atherogenic diets high in cholesterol exhibited significantly reduced survival compared with LDLR knockout mice fed the same diets. In addition to increased diet-accelerated aortic sinus atherosclerosis, we observed significant diet-induced CA atherosclerosis in double knockout mice and diet-dependent accumulation of platelets in CA atherosclerotic plaques. This was accompanied by substantial myocardial fibrosis in double knockout mice fed high cholesterol diets. Atherogenic diet fed double knockout mice also exhibited higher circulating cytokine levels, monocytosis with increased proportions of Ly6C(hi) and Ly6C(int) monocytes, and higher adhesion molecule expression in CA endothelial cells compared with control LDLR knockout mice. CONCLUSIONS:
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Authors | Mark Fuller, Omid Dadoo, Viktoria Serkis, Dina Abutouk, Melissa MacDonald, Neel Dhingani, Joseph Macri, Suleiman A Igdoura, Bernardo L Trigatti |
Journal | Arteriosclerosis, thrombosis, and vascular biology
(Arterioscler Thromb Vasc Biol)
Vol. 34
Issue 11
Pg. 2394-403
(Nov 2014)
ISSN: 1524-4636 [Electronic] United States |
PMID | 25212235
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 American Heart Association, Inc. |
Chemical References |
- Cholates
- Cholesterol, Dietary
- Cytokines
- Receptors, LDL
- Scarb1 protein, mouse
- Scavenger Receptors, Class B
- Vascular Cell Adhesion Molecule-1
- Intercellular Adhesion Molecule-1
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Topics |
- Animals
- Arterial Occlusive Diseases
(epidemiology, metabolism)
- Cholates
(adverse effects)
- Cholesterol, Dietary
(adverse effects)
- Coronary Artery Disease
(epidemiology, metabolism)
- Cytokines
(blood)
- Diet, Atherogenic
(adverse effects)
- Disease Models, Animal
- Endothelium, Vascular
(metabolism)
- Incidence
- Intercellular Adhesion Molecule-1
(metabolism)
- Mice
- Mice, Knockout
- Myocardial Infarction
(epidemiology, metabolism)
- Receptors, LDL
(deficiency, genetics)
- Risk Factors
- Scavenger Receptors, Class B
(deficiency, genetics)
- Vascular Cell Adhesion Molecule-1
(metabolism)
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