Abstract | BACKGROUND/PURPOSE: METHODS: We examined the expression of SEMA3A in paired cancerous and matched adjacent noncancerous gastric mucosa tissues by real-time quantitative RT-PCR (qRT-PCR) and western blotting. In vitro, we evaluate the effects of SEMA3A on gastric cancer cell proliferation and migration by MTT, transwell and wound-healing assays. Furthermore, we analyzed SEMA3A expression in 128 patients who underwent resection procedures using immunohistochemistry. The relationships between the SEMA3A expression levels, the clinicopathological factors, and patient survival were investigated. RESULTS: Our results revealed decreased SEMA3A mRNA (P = 0.0037) and protein (P = 0.033) expression in tumor tissue samples compared with matched adjacent non-tumorous tissue samples. Overexpression of SEMA3A inhibits gastric cancer cell proliferation and migration in vitro. Immunohistochemical staining data showed that SEMA3A expression was significantly decreased in 54.68% of gastric cancer cases. In addition, the chi-square test revealed that low SEMA3A expression was significantly correlated with poor differentiation (P = 0.015), Vascular invasion (P = 0.001), depth of invasion (P < 0.001), lymph node metastasis (P = 0.029), distant metastasis (P = 0.002) and advanced TNM stage (P = 0.003). SEMA3A expression was positively correlated with clinical TNM stage, that suggested the more advanced clinical TNM stage corresponding to the lower expression level of SEMA3A (rs = -0.322, P < 0.001) by Spearman rank correlation analysis. Kaplan-Meier survival analysis demonstrated that low expression of SEMA3A was significantly correlated with a poor prognosis for gastric cancer patients (P < 0.001). The multivariate analysis revealed that SEMA3A expression was an independent prognostic factor of the overall survival rate of patients with gastric cancer. CONCLUSION:
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Authors | Chong Tang, Xuesong Gao, Hongbin Liu, Tian Jiang, Xiaofeng Zhai |
Journal | International journal of clinical and experimental pathology
(Int J Clin Exp Pathol)
Vol. 7
Issue 8
Pg. 4782-94
( 2014)
ISSN: 1936-2625 [Electronic] United States |
PMID | 25197349
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- SEMA3A protein, human
- Semaphorin-3A
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Topics |
- Adenocarcinoma
(metabolism, mortality, pathology)
- Biomarkers, Tumor
(analysis)
- Blotting, Western
- Humans
- Immunohistochemistry
- Kaplan-Meier Estimate
- Prognosis
- Proportional Hazards Models
- Real-Time Polymerase Chain Reaction
- Semaphorin-3A
(biosynthesis)
- Stomach Neoplasms
(metabolism, mortality, pathology)
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