Dual effects of carnosine on energy metabolism of cultured cortical astrocytes under normal and ischemic conditions.

Abstract

OBJECTIVE:

The aim of this study was to investigate the effects of carnosine on the bioenergetic profile of cultured cortical astrocytes under normal and ischemic conditions.

METHODS:

The Seahorse Bioscience XF96 Extracellular Flux Analyzer was used to measure the oxygen consumption rates (OCRs) and extracellular acidification rates (ECARs) of cultured cortical astrocytes treated with and without carnosine under normal and ischemic conditions.

RESULTS:

Under the normal growth condition, the basal OCRs and ECARs of astrocytes were 21.72±1.59 pmol/min/μg protein and 3.95±0.28 mpH/min/μg protein respectively. Mitochondrial respiration accounted for ~80% of the total cellular respiration and 85% of this coupled to ATP synthesis. Carnosine significantly reduced basal OCRs and ECARs and ATP-linked respiration, but it strikingly increased the spare respiratory capacity of astrocytes. The cellular ATP level in carnosine-treated astrocytes was reduced to ~42% of the control. However, under the ischemic condition, carnosine upregulated the mitochondrial respiratory and cellular ATP content of astrocytes exposed to 8h of oxygen-glucose deprivation (OGD) followed by 24 h of recovery under the normal growth condition.

CONCLUSIONS:

Carnosine may be an endogenous regulator of astrocyte energy metabolism and a clinically safe therapeutic agent for promoting brain energy metabolism recovery after ischemia/reperfusion injury.

• Authors: Yao Shen, Yueyang Tian, Jianbo Yang, Xiaojie Shi, Li Ouyang, Jieqiong Gao, Jianxin Lu
• Journal: Regulatory peptides (Regul Pept) 2014 Jun-Aug Vol. 192-193 Pg. 45-52 ISSN: 1873-1686 [Electronic] Netherlands
• PMID: 25195162 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Carnosine

Topics

• Animals
• Astrocytes (cytology, metabolism)
• Brain Ischemia (metabolism)
• Carnosine (metabolism)
• Cells, Cultured
• Energy Metabolism
• Oxygen Consumption
• Rats
• Rats, Sprague-Dawley