Abstract | AIM: MATERIALS & METHODS: RNAi technology was used to silence the expression of VTN in HepG2 and SMMC 7721 cells. Change of growth characteristics in these cells was evaluated. RESULTS: VTN silencing does not affect growth characteristics of cancer cells in monolayer cell culture, but could suppress the colonized growth of cells in soft agar. VTN- siRNA suppresses colony formation more than 80% compared with that of control in SMMC7721cells and leads to the inhibition of colony formation of over 70% in HepG2 cells. In addition, VTN silencing decreases the size of tumor xenografts in nude mice, particularly in male mice, with an inhibition rate of 46.6%. CONCLUSION: VTN plays a significant role in the malignant growth of tumor. Inhibition of VTN could potentially be applied for the treatment of hepatocellular carcinoma.
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Authors | Wei Zhu, Wenxue Li, Guangyu Yang, Chuanxi Fu, Gaofeng Jiang, Qiansheng Hu |
Journal | Future oncology (London, England)
(Future Oncol)
Vol. 11
Issue 2
Pg. 251-8
( 2015)
ISSN: 1744-8301 [Electronic] England |
PMID | 25179307
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Small Interfering
- Vitronectin
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Topics |
- Animals
- Carcinoma, Hepatocellular
(pathology, therapy)
- Cell Proliferation
- Female
- Gene Knockdown Techniques
- Genetic Therapy
- Hep G2 Cells
- Humans
- Liver Neoplasms
(pathology, therapy)
- Male
- Mice, Inbred BALB C
- Mice, Nude
- Neoplasm Transplantation
- RNA Interference
- RNA, Small Interfering
(genetics)
- Vitronectin
(genetics, metabolism)
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