Abstract | BACKGROUND: METHODS: Blood samples were collected from 25 patients with breast cancer at the Kuwait Cancer Control Centre (KCCC). The patients were newly diagnosed, and had not undergone any treatment or surgery at the time of sample collection. The control group consisted of 25 healthy women age-matched (±5 years) to the patients. PBMC were isolated from the patients and controls, and were cultured separately with the mitogen PHA, anti-CD3 antibodies, and Her-2/neu and p53 in the presence or absence of standardized doses of EGCG. The degree of proliferation and interferon-γ [IFN-γ) release were then analyzed. RESULTS: EGCG significantly suppressed the proliferation of PBMC in response to stimulation separately with (i) the mitogen, (ii) anti-CD3, and (iii) the cancer antigen peptides. IFN-γ production was also significantly suppressed by EGCG in vitro. CONCLUSIONS:
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Authors | Farid Saleh, Raj Raghupathy, Sami Asfar, Medhat Oteifa, Noha Al-Saleh |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 14
Pg. 322
(Aug 30 2014)
ISSN: 1472-6882 [Electronic] England |
PMID | 25175005
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Plant Extracts
- Interferon-gamma
- Catechin
- epigallocatechin gallate
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Topics |
- Adult
- Breast Neoplasms
(drug therapy, genetics, metabolism, physiopathology)
- Camellia sinensis
(chemistry)
- Catechin
(analogs & derivatives, pharmacology)
- Cell Proliferation
(drug effects)
- Cells, Cultured
- Female
- Humans
- Interferon-gamma
(genetics, metabolism)
- Leukocytes, Mononuclear
(cytology, drug effects, metabolism)
- Plant Extracts
(pharmacology)
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