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Infection of influenza virus neuraminidase-vaccinated mice with homologous influenza virus leads to strong protection against heterologous influenza viruses.

Abstract
Vaccination is the best measure to prevent influenza pandemics. Here, we studied the protective effect against heterologous influenza viruses, including A/reassortant/NYMC X-179A (pH1N1), A/Chicken/Henan/12/2004 (H5N1), A/Chicken/Jiangsu/7/2002 (H9N2) and A/Guizhou/54/89×A/PR/8/34 (A/Guizhou-X) (H3N2), in mice first vaccinated with a DNA vaccine of haemagglutinin (HA) or neuraminidase (NA) of A/PR/8/34 (PR8) and then infected with the homologous virus. We showed that PR8 HA or NA vaccination both protected mice against a lethal dose of the homologous virus; PR8 HA or NA DNA vaccination and then PR8 infection in mice offered poor or excellent protection, respectively, against a second, heterologous influenza virus challenge. In addition, before the second heterologous influenza infection, the highest antibody level against nucleoprotein (NP) and matrix (M1 and M2) proteins was found in the PR8 NA-vaccinated and PR8-infected group. The level of induced cellular immunity against NP and M1 showed a trend consistent with that seen in antibody levels. However, PR8 HA+NA vaccination and then PR8 infection resulted in limited protection against heterologous influenza virus challenge. Results of the present study demonstrated that infection of the homologous influenza virus in mice already immunized with a NA vaccine could provide excellent protection against subsequent infection of a heterologous influenza virus. These findings suggested that NA, a major antigen of influenza virus, could be an important candidate antigen for universal influenza vaccines.
AuthorsBiao He, Haiyan Chang, Zhihua Liu, Chaoyang Huang, Xueying Liu, Dan Zheng, Fang Fang, Bing Sun, Ze Chen
JournalThe Journal of general virology (J Gen Virol) Vol. 95 Issue Pt 12 Pg. 2627-2637 (Dec 2014) ISSN: 1465-2099 [Electronic] England
PMID25170051 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2014 The Authors.
Chemical References
  • Antibodies, Viral
  • Influenza Vaccines
  • Vaccines, DNA
  • Neuraminidase
Topics
  • Animals
  • Antibodies, Viral
  • Female
  • Immunity, Humoral
  • Influenza A virus (classification, genetics)
  • Influenza Vaccines (immunology)
  • Mice
  • Mice, Inbred BALB C
  • Neuraminidase (immunology)
  • Orthomyxoviridae (classification, enzymology, immunology)
  • Orthomyxoviridae Infections (prevention & control, virology)
  • Reassortant Viruses
  • Specific Pathogen-Free Organisms
  • Vaccines, DNA (immunology)
  • Viral Load

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