B-cell growth-promoting activity in supernatants from CD4+ cells from synovial fluid and peripheral blood of patients with rheumatoid arthritis and other inflammatory arthritides.

Abstract

The purpose of this study was to compare CD4+ cells from peripheral blood (PB) and synovial fluid (SF) of patients with rheumatoid arthritis with regard to mitogen-induced production of B-cell growth-promoting activity. CD4+ cells were isolated by a direct immunomagnetic technique and supernatants from both unstimulated and mitogen-stimulated CD4+ cells were studied. B-cell growth-promoting activity was assayed using highly purified B cells obtained from peripheral blood of healthy individuals. The indicator B cells were isolated by an indirect immunomagnetic technique and solid-phase anti-mu was used for activation of the B cells. Supernatants of unstimulated CD4+ cells from SF and PB did not contain B-cell growth-promoting activity, while usually high levels of B-cell growth-promoting activity were detected in the supernatants from mitogen-stimulated cultures. There was no significant difference in the B-cell growth-promoting activity level between supernatants from SF CD4+ and patient PB CD4+ cells, nor was there any significant difference between SF CD4+ and control PB CD4+ supernatants. The results indicate that the CD4+ cells in the SF have a normal potential for producing B-cell growth-promoting activity.

• Authors: J Hovdenes
• Journal: Scandinavian journal of rheumatology (Scand J Rheumatol) Vol. 18 Issue 6 Pg. 385-92 ( 1989) ISSN: 0300-9742 [Print] England
• PMID: 2515595 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CD4 Antigens
• Interleukin-2
• Interferon-gamma

Topics

• Arthritis (immunology)
• Arthritis, Rheumatoid (immunology)
• B-Lymphocytes (immunology)
• CD4 Antigens (analysis)
• Humans
• Interferon-gamma (biosynthesis)
• Interleukin-2 (metabolism)
• Lymphocyte Activation
• Lymphocytes (immunology, metabolism)
• Synovial Fluid (immunology)