Abstract | OBJECTIVES: METHODS: A total of 662 Swedish children with T1D were matched with 1080 Danish children with T1D and 309 healthy children from Sweden and 283 from Denmark served as controls. Sera were analyzed for the presence of IgA and IgG (IgAG) autoantibodies against deamidated gliadin peptide (DGP) and tissue transglutaminase (tTG) with enzyme-linked immunosorbent assay (ELISA) and IgG-tTG separately in a radioligand binding assay (RBA). Human leukocyte antigen (HLA)-DQB1 and DQA1 genotyping were determined in the T1D cohorts. RESULTS: In the Swedish T1D cohort, 17.2% (114/662) were IgAG-DGP/tTG positive compared with 11.7% (126/1080) in the Danish T1D cohort (p = 0.001) and with 9.4% (29/309) Swedish (p = 0.001) and 5.7% (16/283) Danish (p = 0.003) controls. In the Swedish T1D cohort, both levels of IgAG-DGP/tTG and IgG-tTG were higher compared with the levels in the Danish T1D (p < 0.001). In the control group, 2.8% of the Danish children were positive for both IgAG-DGP/tTG and IgG-tTG, compared to 0.3% of the Swedish. Presence of HLA-DQ2 was equally distributed among 89 children with T1D positive for both IgAG-DGP/tTG and IgG-tTG. CONCLUSION: The discrepancy in levels of IgAG-DGP/tTG and IgG-tTG between Swedish and Danish T1D cohorts was independent of HLA and suggests that regional variations in comorbidity of celiac disease in T1D is caused by difference in exposure to environmental factors.
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Authors | Emma H Adlercreutz, Jannet Svensson, Dorthe Hansen, Karsten Buschard, Åke Lernmark, Henrik Bindesbøl Mortensen, Daniel Agardh |
Journal | Pediatric diabetes
(Pediatr Diabetes)
Vol. 16
Issue 7
Pg. 504-9
(Nov 2015)
ISSN: 1399-5448 [Electronic] Denmark |
PMID | 25131687
(Publication Type: Comparative Study, Journal Article)
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Copyright | © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
- Autoantibodies
- Immunoglobulin A
- Immunoglobulin G
- Peptide Fragments
- Gliadin
- Protein Glutamine gamma Glutamyltransferase 2
- Transglutaminases
- GTP-Binding Proteins
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Topics |
- Adolescent
- Autoantibodies
(analysis)
- Autoimmunity
- Celiac Disease
(blood, complications, epidemiology, immunology)
- Child
- Child, Preschool
- Cohort Studies
- Denmark
(epidemiology)
- Diabetes Mellitus, Type 1
(blood, complications, immunology)
- Female
- GTP-Binding Proteins
(antagonists & inhibitors)
- Gliadin
(antagonists & inhibitors, chemistry)
- Humans
- Immunoglobulin A
(analysis)
- Immunoglobulin G
(analysis)
- Infant
- Male
- Peptide Fragments
(antagonists & inhibitors, metabolism)
- Prevalence
- Protein Glutamine gamma Glutamyltransferase 2
- Retrospective Studies
- Risk
- Sweden
(epidemiology)
- Transglutaminases
(antagonists & inhibitors)
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