Abstract |
Ying-zhi-huang injection (YZH-I) is an injectable multi-herbal prescription derived from the ancient Chinese remedy " Yin-chen-hao-tang", which is widely used in the clinic for the treatment of jaundice and chronic liver diseases. To date, little information is available on the pharmacokinetic properties of this poly-herbal formulation. Herein, we reported a simple, rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantitative multiple reaction monitoring (MRM) of eight major ingredients of YZH-I (including baicalin, baicalein, wogonoside, geniposide, geniposidic acid, chlorogenic acid, neochlorogenic acid, and caffeic acid) in rat plasma. A fast single-tube multi-impurity precipitation extraction ("SMIPE") procedure was introduced for straightforward plasma preparation, based on one-pot deproteinization precipitation with acidified methanol extraction and in-situ multifunction impurity removal by a solid sorbent mixture (anh. magnesium sulfate plus octadecylsilane). Particularly, the addition of ascorbic acid in methanol (10 mg/mL) was found to exhibit a pronounced protective effect and significantly increase extraction effectiveness of the herbal phenolic components. Some pretreatment variables ( protein precipitating solvent, acidifying agent and sorbent) were optimized with acceptable matrix effect (-18 to 7.7%), extraction recovery (65-88%) and process efficiency (62-91%) for the SMIPE-based LC-MRM multi-analyte quantitation using matrix-matched calibration (5-1000 ng/mL) without using internal standard. Mean accuracies were obtained in the range of 83-114% at three different fortification levels, with intra- and inter-day variations within 13%. This validated method was successfully applied to the simultaneous measurement and pharmacokinetic investigation of the chemical constituents in rats following an intravenous administration of YZH-I.
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Authors | Le Zeng, Meiling Wang, Yu Yuan, Bin Guo, Jing Zhou, Zhen Tan, Meiling Ye, Li Ding, Bo Chen |
Journal | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
(J Chromatogr B Analyt Technol Biomed Life Sci)
Vol. 967
Pg. 245-54
(Sep 15 2014)
ISSN: 1873-376X [Electronic] Netherlands |
PMID | 25129410
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier B.V. All rights reserved. |
Chemical References |
- Cinnamates
- Drugs, Chinese Herbal
- Iridoids
- yin zhi huang
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Topics |
- Animals
- Chromatography, Liquid
(methods)
- Cinnamates
(blood, pharmacokinetics)
- Drugs, Chinese Herbal
(administration & dosage, analysis, chemistry, pharmacokinetics)
- Iridoids
(blood, pharmacokinetics)
- Linear Models
- Male
- Rats
- Rats, Sprague-Dawley
- Reproducibility of Results
- Sensitivity and Specificity
- Tandem Mass Spectrometry
(methods)
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