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Molecular cloning, expression analysis and functional characterization of interleukin-22 in So-iny mullet, Liza haematocheila.

Abstract
In the present study, interleukin-22 (IL-22) from So-iny mullet (Liza haematocheila) was identified, and its tissue expression in both healthy and Streptococcus dysgalactiae-infected fish was examined. The full length cDNA sequence of mullet IL-22 was 1070bp, containing an open reading frame of 555bp. The deduced amino acid sequence shared high similarity (45.1-67.9%) with IL-22 from other fish species. Mullet IL-22 also contained an IL-10 family signature and four cysteine residues that were well conserved in other vertebrate IL-22 molecules. Mullet IL-22 mRNA was highly expressed in kidney, moderately expressed in liver and gut, and relatively weakly expressed in spleen, and its expression was significantly up-regulated in all the examined tissues following S. dysgalactiae infection. Furthermore, recombinant mullet IL-22 protein was shown to promote the expression of β-defensin in the four tissues and to increase the survival rate of the fish infected with S. dysgalactiae. Our results suggest mullet IL-22 plays an important role in the immune defense against bacterial infection and has the potential to be used to treat bacterial diseases in fish.
AuthorsZhitao Qi, Qihuan Zhang, Zisheng Wang, Weihong Zhao, Shannan Chen, Qian Gao
JournalMolecular immunology (Mol Immunol) Vol. 63 Issue 2 Pg. 245-52 (Feb 2015) ISSN: 1872-9142 [Electronic] England
PMID25108846 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014 Elsevier Ltd. All rights reserved.
Chemical References
  • DNA, Complementary
  • Interleukins
  • RNA, Messenger
  • Recombinant Proteins
  • beta-Defensins
  • interleukin-22
Topics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary (genetics)
  • Gene Expression Profiling
  • Interleukins (chemistry, genetics, metabolism)
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Messenger (genetics, metabolism)
  • Recombinant Proteins (isolation & purification, pharmacology)
  • Sequence Homology, Amino Acid
  • Smegmamorpha (genetics)
  • Streptococcal Infections (immunology)
  • Streptococcus (drug effects, physiology)
  • Survival Analysis
  • beta-Defensins (genetics, metabolism)

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