Abstract |
In the present study, interleukin-22 (IL-22) from So-iny mullet (Liza haematocheila) was identified, and its tissue expression in both healthy and Streptococcus dysgalactiae-infected fish was examined. The full length cDNA sequence of mullet IL-22 was 1070bp, containing an open reading frame of 555bp. The deduced amino acid sequence shared high similarity (45.1-67.9%) with IL-22 from other fish species. Mullet IL-22 also contained an IL-10 family signature and four cysteine residues that were well conserved in other vertebrate IL-22 molecules. Mullet IL-22 mRNA was highly expressed in kidney, moderately expressed in liver and gut, and relatively weakly expressed in spleen, and its expression was significantly up-regulated in all the examined tissues following S. dysgalactiae infection. Furthermore, recombinant mullet IL-22 protein was shown to promote the expression of β- defensin in the four tissues and to increase the survival rate of the fish infected with S. dysgalactiae. Our results suggest mullet IL-22 plays an important role in the immune defense against bacterial infection and has the potential to be used to treat bacterial diseases in fish.
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Authors | Zhitao Qi, Qihuan Zhang, Zisheng Wang, Weihong Zhao, Shannan Chen, Qian Gao |
Journal | Molecular immunology
(Mol Immunol)
Vol. 63
Issue 2
Pg. 245-52
(Feb 2015)
ISSN: 1872-9142 [Electronic] England |
PMID | 25108846
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Ltd. All rights reserved. |
Chemical References |
- DNA, Complementary
- Interleukins
- RNA, Messenger
- Recombinant Proteins
- beta-Defensins
- interleukin-22
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Topics |
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Complementary
(genetics)
- Gene Expression Profiling
- Interleukins
(chemistry, genetics, metabolism)
- Molecular Sequence Data
- Phylogeny
- RNA, Messenger
(genetics, metabolism)
- Recombinant Proteins
(isolation & purification, pharmacology)
- Sequence Homology, Amino Acid
- Smegmamorpha
(genetics)
- Streptococcal Infections
(immunology)
- Streptococcus
(drug effects, physiology)
- Survival Analysis
- beta-Defensins
(genetics, metabolism)
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