Abstract |
Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied. Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved. EPS synthesis and cell growth had different optima. For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %). As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc) content in EPS increased, while galactose, xylose, and glucose decreased. Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS. Aeration intensity and time of cultivation had little effect on EPS composition. Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352. The row EPS was composed of acidic glucuronoxylomannan for more than 85 %. In the in vivo experiments, EPS significantly improved excisional wound healing in healthy rats. The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
|
Authors | Dzianis Smirnou, Dana Hrubošová, Jaromír Kulhánek, Karol Švík, Lenka Bobková, Veronika Moravcová, Martin Krčmář, Lukáš Franke, Vladimír Velebný |
Journal | Applied biochemistry and biotechnology
(Appl Biochem Biotechnol)
Vol. 174
Issue 4
Pg. 1344-1353
(Oct 2014)
ISSN: 1559-0291 [Electronic] United States |
PMID | 25106895
(Publication Type: Journal Article)
|
Chemical References |
|
Topics |
- Animals
- Cryptococcus
(growth & development, metabolism)
- Fungal Polysaccharides
(biosynthesis, pharmacology)
- Rats
- Wound Healing
(drug effects)
- Wounds and Injuries
(drug therapy)
|