HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Vitamin A and feeding statuses modulate the insulin-regulated gene expression in Zucker lean and fatty primary rat hepatocytes.

Abstract
Unattended hepatic insulin resistance predisposes individuals to dyslipidemia, type 2 diabetes and many other metabolic complications. The mechanism of hepatic insulin resistance at the gene expression level remains unrevealed. To examine the effects of vitamin A (VA), total energy intake and feeding conditions on the insulin-regulated gene expression in primary hepatocytes of Zucker lean (ZL) and fatty (ZF) rats, we analyze the expression levels of hepatic model genes in response to the treatments of insulin and retinoic acid (RA). We report that the insulin- and RA-regulated glucokinase, sterol regulatory element-binding protein-1c and cytosolic form of phosphoenolpyruvate carboxykinase expressions are impaired in hepatocytes of ZF rats fed chow or a VA sufficient (VAS) diet ad libitum. The impairments are partially corrected when ZF rats are fed a VA deficient (VAD) diet ad libitum or pair-fed a VAS diet to the intake of their VAD counterparts in non-fasting conditions. Interestingly in the pair-fed ZL and ZF rats, transient overeating on the last day of pair-feeding regimen changes the expression levels of some VA catabolic genes, and impairs the insulin- and RA-regulated gene expression in hepatocytes. These results demonstrate that VA and feeding statuses modulate the hepatic insulin sensitivity at the gene expression level.
AuthorsWei Chen, Meredith L Howell, Yang Li, Rui Li, Guoxun Chen
JournalPloS one (PLoS One) Vol. 9 Issue 8 Pg. e100868 ( 2014) ISSN: 1932-6203 [Electronic] United States
PMID25105869 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Insulin
  • Tretinoin
  • Phosphoenolpyruvate Carboxykinase (ATP)
Topics
  • Analysis of Variance
  • Animals
  • Dietary Supplements
  • Gene Expression Regulation (drug effects, physiology)
  • Hepatocytes (drug effects, metabolism)
  • Insulin (pharmacology)
  • Insulin Resistance (physiology)
  • Male
  • Phosphoenolpyruvate Carboxykinase (ATP) (metabolism)
  • Rats
  • Rats, Zucker (metabolism, physiology)
  • Real-Time Polymerase Chain Reaction
  • Tretinoin (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: