Modulation by IFN-gamma of the metastatic ability of murine, human, and H-2-transfected tumor cells.

Abstract

Interferon-gamma (IFN-gamma) can enhance the experimental metastatic ability of B16 melanoma. The in vitro treatment with IFN-gamma of four clones derived from the murine mammary adenocarcinoma TS/A increased the number of lung colonies observed after intravenous injection in syngeneic mice. The spontaneous metastatic ability of these clones was not altered by the IFN-gamma pretreatment nor by daily intratumor injection of low-dose IFN-gamma. The experimental metastatic ability in nude mice of the human rhabdomyosarcoma cell line RD was decreased by in vitro pretreatment with IFN-gamma. To study the role played by major histocompatibility complex gene products in the IFN-gamma-mediated enhancement of B16 experimental metastasis, a mutant B16 clone, B78H1, was transfected with the H-2Kb gene. B78H1 cells are not capable of expressing H-2b even after treatment with IFN-gamma; IFN-gamma readily induced high levels of H-2Kb in a set of transfected clones, but did not enhance their experimental metastatic ability.

• Authors: P L Lollini, C De Giovanni, G Nicoletti, K Scotlandi, L Landuzzi, P Nanni
• Journal: Tumori (Tumori) Vol. 75 Issue 4 Pg. 383-8 (Aug 31 1989) ISSN: 0300-8916 [Print] United States
• PMID: 2510384 (Publication Type: Journal Article)

Chemical References

• H-2 Antigens
• Recombinant Proteins
• Interferon-gamma

Topics

• Animals
• H-2 Antigens (genetics)
• Humans
• Interferon-gamma (pharmacology)
• Mammary Neoplasms, Animal (pathology)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Neoplasm Metastasis
• Recombinant Proteins
• Rhabdomyosarcoma (pathology)
• Transfection
• Tumor Cells, Cultured